Evaluation of the Antibacterial Efficacy of Silver Nanoparticles against Enterococcus faecalis Biofilm
Evaluation of the Antibacterial Efficacy of Silver Nanoparticles against Enterococcus faecalis Biofilm
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纳米银粒子对粪肠球菌生物膜的抗菌效果评价
DOI：
10.1016/j.joen.2013.08.022
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发表时间：
2014-02-01
影响因子：
4.2
通讯作者：
Fan, Bing
中科院分区：
文献类型：
--
作者：
Wu, Daming;Fan, Wei;Fan, Bing
简介：本研究的目的是评估纳米银颗粒 (AgNP) 作为冲洗剂或药物对根部牙本质上形成的粪肠球菌生物膜的抗菌功效。方法：牙本质切片接种粪肠球菌4周，建立标准单种生物膜模型。这些生物膜分两个阶段进行测试。在第1阶段，分别用0.1％AgNP溶液、2％次氯酸钠和无菌盐水冲洗生物膜2分钟。在第 2 阶段，生物膜用 AgNP 凝胶（0.02% 和 0.01%）和氢氧化钙处理 7 天。使用扫描电子显微镜评估每组一半标本的超微结构，而使用共聚焦激光扫描显微镜结合活力染色评估另一半标本的活菌结构和分布。结果：0.1%AgNP溶液注射器冲洗并未破坏生物膜结构，生物膜结构中活菌比例与生理盐水组无差异(P>.05)，但低于对照组(P<.05)。用0.02% AgNP凝胶作为药物处理的生物膜显着破坏了生物膜的结构完整性，并且导致后处理次数最少。与 0.01% AgNP 凝胶和氢氧化钙组相比，残留的活粪肠球菌细胞 (P < .05)。结论：本研究的结果表明，AgNP 的抗生物膜功效取决于应用方式。 AgNP 作为药物而非冲洗剂显示出在根管消毒过程中消除残留细菌生物膜的潜力。
Introduction: The purpose of this study was to evaluate the antibacterial efficacy of silver nanoparticles (AgNPs) as an irrigant or medicament against Enterococcus faecalis biofilms formed on root dentin. Methods: Dentin sections were inoculated with E. faecalis for 4 weeks to establish a standard monospecies biofilm model. These biofilms were tested in 2 stages. In stage 1, the biofilms were irrigated with 0.1% AgNP solution, 2% sodium hypochlorite, and sterile saline for 2 minutes, respectively. In stage 2, the biofilms were treated with AgNP gel (0.02% and 0.01%) and calcium hydroxide for 7 days. The ultrastructure of one half of the specimens from each group was evaluated by using scanning electron microscopy, whereas the structure and distribution of viable bacteria of the other half of the specimens were assessed with confocal laser scanning microscopy combined with viability staining. Results: Syringe irrigation with 0.1% AgNP solution did not disrupt the biofilm structure, and the proportion of viable bacteria in the biofilm structures was not different from that of the saline group (P > .05) but was less than that of the control group (P < .05). The biofilms treated with 0.02% AgNP gel as medicament significantly disrupted the structural integrity of the biofilm and resulted in the least number of post-treatment. residual viable E. faecalis cells compared with 0.01% AgNP gel and calcium hydroxide groups (P < .05). Conclusions: The findings from this study suggested that the antibiofilm efficacy of AgNPs depends on the mode of application. AgNPs as a medicament and not as an irrigant showed potential to eliminate residual bacterial biofilms during root canal disinfection.