Changes in major capsid genes (g23) of T4-type bacteriophages with soil depth in two Japanese rice fields
Changes in major capsid genes (g23) of T4-type bacteriophages with soil depth in two Japanese rice fields
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DOI：
10.1007/s00374-009-0362-2
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发表时间：
2009-02
影响因子：
6.5
通讯作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura
中科院分区：
文献类型：
--
作者：
Guanghua Wang;J. Murase;K. Taki;Yoshinori Ohashi;N. Yoshikawa;S. Asakawa;M. Kimura
虽然众所周知，土壤中的微生物群落会随着土壤深度的变化而变化，但病毒群落随土壤深度的变化还没有记录在案。对两个日本稻田T4型噬菌体群落的土壤深度剖面进行了研究，结果表明，T4型噬菌体群落随土壤深度的变化而变化，可分为两类：水稻根系密集发育的表层群落和深层群落。尽管土壤质地在两个剖面的底土层中都是分层的，但变性梯度凝胶电泳谱和G23在两个田的底土层之间的系统发育相似性很高，表明土壤质地不影响这些田的T4型噬菌体群落。此外，一些克隆的gg23序列与从东北中国地区稻田中获得的序列完全相同，这表明密切相关的病毒及其寄主分布在日本和中国东北地区之间的海域。
Although microbial communities in soil are well known to change with soil depth, the changes in viral communities with soil depth have not been documented. This study examined the soil depth profiles of T4-type phage communities in two Japanese rice fields fromg23clones in soil DNA extracts to a depth of 1 m. T4-type phage communities changed with soil depth, and the communities were grouped into two groups: the communities of the surface soil layers, where rice roots developed densely, and those of the subsoil layers. Although coarse- and fine-textured soils were stratified in the subsoil layers in both profiles, denaturing gradient gel electrophoresis band patterns and phylogenetic affiliation ofg23were highly similar to each other among the subsoil layers in both fields, indicating that soil texture did not affect T4-type phage communities in these fields. In addition, some clones hadg23sequences identical to those retrieved from rice fields in Northeast China, indicating that closely related viruses and their hosts distribute across the sea between rice fields in Japan and Northeast China.