Changes in the transcriptomic profiles of maize roots in response to iron-deficiency stress
Changes in the transcriptomic profiles of maize roots in response to iron-deficiency stress
复制标题
缺铁胁迫下玉米根转录组谱的变化
DOI：
10.1007/s11103-014-0189-6
复制
发表时间：
2014-07-01
影响因子：
5.1
通讯作者：
Zhang, Chunqing
中科院分区：
文献类型：
--
作者：
Li, Yan;Wang, Nian;Zhang, Chunqing
铁（Fe）的低溶解性使植物经常遭受缺铁胁迫。植物已经进化出两种不同的策略来溶解和运输铁以适应这种非生物胁迫条件。利用Illumina数字基因表达进行转录组学分析，以了解玉米（一种重要的策略II植物）根系对Fe饥饿的抗性反应的机制。在缺铁处理1、2、4或7天后，分别有3，427、4，069、4，881和2，610个基因的表达水平发生了显著变化。2′-脱氧mugineic酸（DMA）合成、分泌和Fe（III）-DMA摄取相关基因被显著诱导。许多与植物激素、蛋白激酶和蛋白磷酸酶相关的基因对缺铁胁迫有反应，表明它们在缺铁胁迫中起着调节作用。功能注释聚类分析，使用数据库的注释，可视化和集成发现，揭示了玉米根对铁饥饿的反应。这导致了38个功能注释簇：25个为上调基因，13个为下调基因。这些基因包括编码参与羧酸、类异戊二烯和芳香族化合物代谢的酶、转运蛋白和应激反应蛋白的基因。本研究为了解玉米对缺铁胁迫的反应提供了综合信息。
Plants are often subjected to iron (Fe)-deficiency stress because of its low solubility. Plants have evolved two distinct strategies to solubilize and transport Fe to acclimate to this abiotic stress condition. Transcriptomic profiling analysis was performed using Illumina digital gene expression to understand the mechanism underlying resistance responses of roots to Fe starvation in maize, an important Strategy II plant. A total of 3,427, 4,069, 4,881, and 2,610 genes had significantly changed expression levels after Fe-deficiency treatments of 1, 2, 4 or 7 days, respectively. Genes involved in 2′-deoxymugineic acid (DMA) synthesis, secretion, and Fe(III)–DMA uptake were significantly induced. Many genes related to plant hormones, protein kinases, and protein phosphatases responded to Fe-deficiency stress, suggesting their regulatory roles in response to the Fe-deficiency stress. Functional annotation clustering analysis, using the Database for Annotation, Visualization and Integrated Discovery, revealed maize root responses to Fe starvation. This resulted in 38 functional annotation clusters: 25 for up-regulated genes, and 13 for down-regulated ones. These included genes encoding enzymes involved in the metabolism of carboxylic acids, isoprenoids and aromatic compounds, transporters, and stress response proteins. Our work provides integrated information for understanding maize response to Fe-deficiency stress.