PROTEIN SYNTHESIS IN RAT LIVER - INFLUENCE OF AMINO ACIDS IN DIET ON MICROSOMES AND POLYSOMES
PROTEIN SYNTHESIS IN RAT LIVER - INFLUENCE OF AMINO ACIDS IN DIET ON MICROSOMES AND POLYSOMES
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DOI：
10.1126/science.150.3696.628
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发表时间：
1965-01-01
期刊：
影响因子：
56.9
通讯作者：
MUNRO, HN
中科院分区：
文献类型：
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作者：
FLECK, A;SHEPHERD, J;MUNRO, HN
大鼠经胃管饲喂营养完全或缺乏色氨酸的氨基酸混合物1小时后处死。从喂食不完全混合物的动物肝脏中分离的微粒体在体外吸收c14 -亮氨酸的能力降低，而从同一肝脏制备的多聚体显示出单体和二体的比例增加。先前用放线菌素D处理并不能阻止对两种氨基酸混合物的反应差异，这表明信使核糖核酸（RNA）的合成可能不参与识别氨基酸模式差异的细胞机制。
Rats were killed 1 hour after having been fed by stomach tube with amino acid mixtures that were either nutritionally complete or lacked tryptophan. Microsomes isolated from the livers of animals fed the incomplete mixture showed a reduced capacity to incorporate C14-leucine in vitro, and polysomes prepared from the same livers showed an increased proportion of monosomes and disomes. Prior treatment with actinomycin D did not prevent these differences in response to the 2 amino acid mixtures, an indication that synthesis of messenger ribonucleic acid (RNA) may not be involved in the cell mechanism which recognizes differences in amino acid pattern.