Treatment with Bifidobacterium bifidum 17 partially protects mice from Th1-driven inflammation in a chemically induced model of colitis
Treatment with Bifidobacterium bifidum 17 partially protects mice from Th1-driven inflammation in a chemically induced model of colitis
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DOI：
10.1016/j.ijfoodmicro.2010.12.020
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发表时间：
2011-09-01
影响因子：
5.4
通讯作者：
Riedel, Christian U.
中科院分区：
文献类型：
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作者：
Philippe, David;Heupel, Esther;Riedel, Christian U.
益生菌已被建议作为一种替代治疗方法在干预胃肠道炎症性疾病（GIT）。应用单一菌株或益生菌混合物在动物模型和炎症性肠病（IBD）患者中显示出有希望的结果。我们最近在使用肠上皮细胞的细胞培养模型中证明了两歧双歧杆菌517对lps诱导的炎症事件的有效抑制能力，并在两种结肠炎小鼠模型中验证了这些抗炎作用。在本研究中，我们分析了这种潜在的益生菌菌株在化学诱导的C57BL/6小鼠结肠炎模型中的抗炎作用。该模型的特点是强烈的1T辅助（Th1）反应类似于克罗恩病，这是两种最常见的IBD形式之一。我们进行了宏观分析，并确定了两歧双歧杆菌517对结肠粘膜活组织检查中细胞因子平衡的影响。两歧双歧杆菌S17对体重减轻只有边际效应，而宏观参数没有观察到差异。然而，组织学评分和促炎细胞因子白细胞介素1 β (IL-1 β)、白细胞介素6 （IL-6）、角化细胞来源的趋化因子（KC）以及炎症标志物环氧化酶2 （Cox-2）和髓过氧化物酶（MPO）水平显著降低。这些结果表明，两歧双歧杆菌517治疗能够部分抑制在我们的结肠炎模型中引起的强烈的th1驱动的肠道炎症。(C) 2010 Elsevier B.V.版权所有
Probiotics have been suggested as an alternative therapeutical approach in the intervention of inflammatory disorders of the gastrointestinal tract (GIT). Application of single strains or probiotic mixtures has shown promising results in animal models and patients of inflammatory bowel disease (IBD). We recently demonstrated potent inhibitory capacity of a Bifidobacterium bifidum 517 on LPS-induced inflammatory events in cell culture models using intestinal epithelial cells and verified these anti-inflammatory effects in two mouse models of colitis. In the present study we analyze the anti-inflammatory effect of this potential probiotic strain in a chemically-induced model of colitis in C57BL/6 mice. This model is characterized by a strong type 1T helper (Th1) response resembling Crohn's disease, one of the two most prevalent forms of IBD. We performed macroscopic analysis and determined the effect of B. bifidum 517 on the cytokine balance in biopsies of the colonic mucosa. While treatment with B. bifidum S17 only had a marginal effect on weight loss, no difference was observed in the macroscopic parameters. However, a significant reduction in histology scores and the levels of pro-inflammatory cytokines interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6), keratinocyte-derived chemokine (KC) and the inflammatory markers cyclooxigenase 2 (Cox-2) and myeloperoxidase (MPO) was observed. These results indicate that treatment with B. bifidum 517 is able to partially inhibit the strong Th1-driven intestinal inflammation induced in our model of colitis. (C) 2010 Elsevier B.V. All rights reserved.