Dose response relationship of allergen, histamine, and histamine releasers in skin prick test and precision of the skin prick test method
Dose response relationship of allergen, histamine, and histamine releasers in skin prick test and precision of the skin prick test method
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皮肤点刺试验中过敏原、组胺和组胺释放剂的剂量反应关系及皮肤点刺试验方法的精密度
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发表时间：
1987
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通讯作者：
O. Zetterström
中科院分区：
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作者：
S. Dreborg;M. Holgersson;G. Nilsson;O. Zetterström
本研究的目的是研究皮肤点刺试验（SPT）中过敏原、组胺和组胺释放剂的剂量反应关系以及SPT方法的精确度。在一项实验中，对 7 名患者研究了蒂莫西过敏原、盐酸组胺、兔抗人 IgE 和化合物 48/80。在第二个实验中，对两组 10 名患者进行了蒂莫西过敏原和组胺以及狗过敏原和组胺的研究。组胺HCl 1 和10 mg/ml 分别诱导约15 和25 mm2（直径4.5 和5.5 mm）的疤痕。对于大于 10 mm2 的疤痕面积，以变异系数表示的精确度对于组胺约为 25%，对于过敏原约为 40%。用于测试剂量反应关系的回归线的计算基于最小二乘法。最适合的是对数/对数模型。患者体内过敏原、组胺和组胺释放剂的斜率基本上是平行的。根据伤痕面积估计过敏原的中位斜率约为 0.4，根据平均伤痕直径估计为 0.2。此外，尽管在较低浓度下存在斜率更陡的趋势，但过敏原和组胺的剂量反应曲线的下部和上部之间没有发现显着差异。这些结果表明，组胺浓度 1 mg/ml 应用作 SPT 中的阳性对照，并且组胺释放剂在 SPT 中作为参考物质并不比组胺更具优势。过敏原和组胺的剂量反应关系的共同斜率可用于估计皮肤敏感性。
The aim of the present investigation was to study the dose response relationship of allergen, histamine and histamine releasers in skin prick test (SPT) and the precision of the SPT method. In one experiment timothy allergen, histamine HCl, rabbit anti‐human‐IgE and compound 48/80 were studied in seven patients. In a second experiment timothy allergen and histamine and dog allergen and histamine were investigated in two groups of 10 patients. Histamine HCl 1 and 10 mg/ml induced weals about 15 and 25 mm2 (4.5 and 5.5 mm in diameter), respectively. The precision as expressed by the coefficient of variation was about 25 % for histamine and 40 % for allergen for weal areas > 10 mm2. Calculations of the regression lines to test the dose response relationships were based on the method of least squares. The best fit was to a log/log model. The slopes of allergen, histamine and histamine releasers were essentially parallel within patients. The median slope of allergen was estimated to about 0.4 based on weal areas and 0.2 based on mean weal diameters. Furthermore, no significant differences were found between the lower and upper parts of the dose response curves of allergen and histamine, although there was a tendency towards steeper slopes at lower concentrations. These results show that histamine concentrations 1 mg/ml should be used as positive control in SPT and that histamine releasers do not offer advantages over histamine as reference substances in SPT. A common slope for the dose response relationship of allergen and histamine can be used for the estimation of skin sensitivity.