Multiplex PCR and multicolor probes melting for the simultaneous detection of five UGT1A1 variants
Multiplex PCR and multicolor probes melting for the simultaneous detection of five UGT1A1 variants
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DOI：
10.1016/j.ab.2019.113448
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发表时间：
2019-12-15
影响因子：
2.9
通讯作者：
Matsushita, Kazuyuki
中科院分区：
文献类型：
--
作者：
Ishige, Takayuki;Itoga, Sakae;Matsushita, Kazuyuki
建立了多重PCR熔融分析方法，用于检测UGT1A1的5个变异。使用颜色探针和T-m实现多路复用。*28/*6、*27、*29、*7探针用颜色区分。虽然*28和*6的探针颜色相同，但探针T-m明显区分了它们的变体。各基因型等位基因频率分别为*28 0.12、*6 0.19、*27 0.02、*29 0.0、*7 0.005。我们建立了一种多重PCR熔融分析方法，可用于临床实验室的分子诊断和药物遗传学分析。
The multiplex PCR melting analysis method was developed for detecting the five UGT1A1 variants. Multiplexing was achieved using color probes and T-m. The probes for *28/*6, *27, *29, and *7 were discriminated by colors. Although the probes for *28 and *6 had the same colors, their variants were clearly discriminated by probe T-m. The allelic frequencies of each genotype were 0.12 for *28, 0.19 for *6, 0.02 for *27, 0.0 for *29, and 0.005 for *7. We developed a multiplex PCR melting analysis method, which will be useful in molecular diagnostics and pharmacogenetic analyses in clinical laboratories.