Preferential cleavage of S-S and C-S bonds in electron detachment dissociation and infrared multiphoton dissociation of disulfide-linked peptide anions
Preferential cleavage of S-S and C-S bonds in electron detachment dissociation and infrared multiphoton dissociation of disulfide-linked peptide anions
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DOI：
10.1016/j.ijms.2007.01.001
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发表时间：
2007-05-15
影响因子：
1.8
通讯作者：
Hakansson, Kristina
中科院分区：
文献类型：
--
作者：
Kalli, Anastasia;Hakansson, Kristina
二硫键通常在正离子模式碰撞活化解离（CAD）中仅显示有限的裂解。然而，已经证明，在负离子模式中存在相反的情况，其中优先发生S-S和C-S键断裂。在这里，我们表明，电子分离解离（EDD）和红外多光子解离（IRMPD）的肽阴离子含有二硫键也导致优先裂解的S-S和C-S键。所得产物主要是EDD中的自由基离子，而IRMPD产生偶数电子产物离子，正如预期的那样，从而支持这两种裂解过程的不同二硫键裂解机制。我们还表明，在EDD中，色氨酸的存在会导致大量侧链丢失（129 Da），这可以有效地与二硫键切割竞争。(c)2007年爱思唯尔B。V.保留所有权利。
Disulfide bonds generally show only limited cleavage in positive ion mode collision activated dissociation (CAD). However, it has been demonstrated that a reverse situation exists in negative ion mode in which preferential S-S and C-S bond cleavage occurs. Here, we show that electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) of peptide anions containing disulfide linkages also result in preferential cleavage of S-S and C-S bonds. Resulting products are mainly radical ions in EDD whereas IRMPD produces even-electron product ions, as expected, thereby supporting different disulfide cleavage mechanisms for these two fragmentation processes. We also show that, in EDD, the presence of tryptophan can result in abundant side chain loss (129 Da), which effectively can compete with disulfide bond cleavage. (c) 2007 Elsevier B. V. All rights reserved.