Transformation-related expression of a low-molecular-mass tropomyosin isoform TM5/TM30nm in transformed rat fibroblastic cell lines
Transformation-related expression of a low-molecular-mass tropomyosin isoform TM5/TM30nm in transformed rat fibroblastic cell lines
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DOI：
10.1007/bf01438309
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发表时间：
1997-06-01
影响因子：
3.6
通讯作者：
Taniguchi, S
中科院分区：
文献类型：
--
作者：
Miyado, K;Sato, M;Taniguchi, S
我们克隆了大鼠TM 5/TM 30 nm基因的全长cDNA。以该cDNA为探针，我们证实TM 5/TM 30 nm mRNA在致瘤大鼠成纤维细胞系SR-3 Y1 -2和fos-SR-3 Y1 -202中的表达高于正常细胞系3 Y1。用抗TM 5/TM 30 nm抗血清进行Western blot分析，结果显示TM 5/TM 30 nm蛋白在SR-3 Y1 -2和fos-SR-3 Y1 -202细胞中有高表达。此外，该蛋白的细胞定位在3 Y1细胞和致瘤细胞之间不同。这些结果表明，TM 5/TM 30 nm参与大鼠成纤维细胞的恶性转化。
We cloned a full-length rat TM5/TM30nm cDNA. Using this cDNA as a probe, we demonstrated that expression of TM5/TM30nm mRNA was higher in the tumorigenic rat fibroblastic cell lines SR-3Y1-2 and fos-SR-3Y1-202 than in the normal cell line 3Y1. High expression of TM5/TM30nm protein in SR-3Y1-2 and fos-SR-3Y1-202 cells was also detected by Western blot analysis using anti-TM5/TM30nm antiserum. In addition, the cellular localization of this protein differed between 3Y1 cells and tumorigenic ones. These findings suggest that TM5/TM30nm is involved in malignant transformation of rat fibroblastic cells.