ANTIBODIES TO CARBONIC-ANHYDRASE IN PATIENTS WITH IMMUNE CHOLANGIOPATHIES
ANTIBODIES TO CARBONIC-ANHYDRASE IN PATIENTS WITH IMMUNE CHOLANGIOPATHIES
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DOI：
10.1016/0016-5085(95)90143-4
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发表时间：
1995-06-01
期刊：
影响因子：
29.4
通讯作者：
KIECHLE, FL
中科院分区：
文献类型：
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作者：
GORDON, SC;QUATTROCIOCCHILONGE, TM;KIECHLE, FL
背景/目的：胆管上皮含有丰富的碳酸酐酶。这种酶的抗体已在自身免疫性疾病中得到描述。研究了免疫介导性肝病患者的血清，以确定碳酸酐酶 II 和/或丙酮酸脱氢酶抗体是否可以在免疫学上区分自身免疫性胆管炎与原发性胆汁性肝硬化。方法：对自身免疫性胆管炎 (6)、原发性胆汁性肝硬化 (12)、原发性硬化性胆管炎 (12)、自身免疫性肝炎 (12) 和对照（吉尔伯特综合征；8）患者的血清进行碳酸酐酶 II（蛋白质印迹）和丙酮酸脱氢酶（流式细胞术）抗体测定。结果：6 名自身免疫性胆管炎患者中 5 名检测到碳酸酐酶 II 反应性，12 名原发性胆汁性肝硬化患者中 1 名检测到碳酸酐酶 II 反应性，12 名自身免疫性肝炎患者中 1 名检测到碳酸酐酶 II 反应性，其他患者均未检测到碳酸酐酶 II 反应性。患有自身免疫性胆管炎的个体比其他患者更有可能对碳酸酐酶II产生反应(P<0.001)。与所有其他组相比，原发性胆汁性肝硬化患者对丙酮酸脱氢酶的反应性更高（P < 0.001）。结论：人碳酸酐酶 II 抗体经常在自身免疫性胆管炎患者的血清中检测到，而在其他胆管病中不常见或不存在。这些数据提供的证据表明，自身免疫性胆管炎和原发性胆汁性肝硬化代表具有独特免疫反应模式的不同实体。
Background/Aims: Bile duct epithelia contain an abundance of carbonic anhydrase. Antibodies to this enzyme have been described in autoimmune disorders. Serum from patients with immune-mediated liver diseases was studied to determine whether antibodies to carbonic anhydrase II and/or pyruvate dehydrogenase could distinguish autoimmune cholangitis as immunologically distinct from primary biliary cirrhosis. Methods: Antibody assays to carbonic anhydrase II (Western blot) and pyruvate dehydrogenase (flow cytometry) were performed on the sera of patients with autoimmune cholangitis (6), primary biliary cirrhosis (12), primary sclerosing cholangitis (12), autoimmune hepatitis (12), and control (Gilbert syndrome; 8). Results: Reactivity to carbonic anhydrase II was detected in 5 of 6 patients with autoimmune cholangitis, 1 of 12 patients with primary biliary cirrhosis, 1 of 12 patients with autoimmune hepatitis, and no other patients. Individuals with autoimmune cholangitis were more likely than the other patients to be reactive to carbonic anhydrase Il (P < 0.001). Patients with primary biliary cirrhosis were more reactive to pyruvate dehydrogenase compared with all other groups (P < 0.001). Conclusions: An antibody to human carbonic anhydrase II is frequently detected in the sera of patients with autoimmune cholangitis and is uncommon or not present in other cholangiopathies. These data provide evidence that autoimmune cholangitis and primary biliary cirrhosis represent distinct entities with unique patterns of immunoreactivity.