Measurement of regional rates of cerebral protein synthesis with L-[1-11C]leucine and PET with correction for recycling of tissue amino acids: II. Validation in rhesus monkeys.
Measurement of regional rates of cerebral protein synthesis with L-[1-11C]leucine and PET with correction for recycling of tissue amino acids: II. Validation in rhesus monkeys.
复制标题
用L-[1-11C]亮氨酸和PET测量脑蛋白合成的区域速率并校正组织氨基酸的再循环：II。
DOI：
10.1038/sj.jcbfm.9600066
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Vuong,Bik-Kee
中科院分区：
文献类型：
--
作者：
Smith,CarolynBeebe;Schmidt,KathleenC;Qin,Mei;Burlin,ThomasV;Cook,MichelleP;Kang,Julia;Saunders,RichardC;Bacher,JohnD;Carson,RichardE;Channing,MichaelA;Eckelman,WilliamC;Herscovitch,Peter;Laverman,Peter;Vuong,Bik-Kee
The confounding effect of recycling of amino acids derived from tissue protein breakdown into the precursor pool for protein synthesis has been an obstacle to adaptingin vivomethods for determination of regional rates of cerebral protein synthesis (rCPS) to positron emission tomography (PET). We used a kinetic modeling approach to estimate λ, the fraction of the precursor pool for protein synthesis derived from arterial plasma, and to measure rCPS in three anesthetized adult monkeys dynamically scanned after a bolus injection of L-[1-11C]leucine. In the same animals, λ was directly measured in a steady-state terminal experiment, and values showed excellent agreement with those estimated in the PET studies. In three additional monkeys rCPS was determined with the quantitative autoradiographic L-[1-14C]leucine method. In whole brain and cerebellum, rates of protein synthesis determined with the autoradiographic method were in excellent agreement with those determined with PET, and regional values were in good agreement when differences in spatial resolution of the two methods were taken into account. Low intrasubject variability was found on repeated PET studies. Our results in anesthetized monkey indicate that, by using a kinetic modeling approach to correct for recycling of tissue amino acids, quantitatively accurate and reproducible measurement of rCPS is possible with L-[1-11C]leucine and PET.
