Goα and diacylglycerol kinase negatively regulate the Gqα pathway in C-elegans
Goα and diacylglycerol kinase negatively regulate the Gqα pathway in C-elegans
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DOI：
10.1016/s0896-6273(00)80847-8
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发表时间：
1999-10-01
期刊：
影响因子：
16.2
通讯作者：
Rand, JB
中科院分区：
文献类型：
--
作者：
Miller, KG;Emerson, MD;Rand, JB
我们研究了C.通过使用遗传筛选来鉴定赋予与EGL-30突变体相似的表型的基因。一个这样的基因，egl-8，编码磷脂酶CP，存在于整个神经系统和肠细胞连接附近。EGL-30和EGL-8似乎积极调节突触传递，因为降低其功能会导致强烈的涕灭威抗性和缓慢的运动速率。相比之下，果阿-1（G（o）alpha）和DGK-1（二酰基甘油激酶）似乎对突触传递起负调节作用，因为降低它们的功能会导致强烈的涕灭威超敏反应和过度活跃的运动。遗传分析表明，果阿-1负调节EGL-30途径，而DGK-1拮抗EGL-30途径。
We investigated the EGL-30 (G(q)alpha) pathway in C. elegans by using genetic screens to identify genes that confer phenotypes similar to egl-30 mutants. One such gene, egl-8, encodes a phospholipase CP that is present throughout the nervous system and near intestinal cell junctions. EGL-30 and EGL-8 appear to positively regulate synaptic transmission because reducing their function results in strong aldicarb resistance and slow locomotion rates. In contrast, GOA-1 (G(o)alpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate synaptic transmission, because reducing their function results in strong aldicarb hypersensitivity and hyperactive locomotion. A genetic analysis suggests that GOA-1 negatively regulates the EGL-30 pathway and that DGK-1 antagonizes the EGL-30 pathway.