Metre-long cell-laden microfibres exhibit tissue morphologies and functions
Metre-long cell-laden microfibres exhibit tissue morphologies and functions
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DOI：
10.1038/nmat3606
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发表时间：
2013-06-01
期刊：
影响因子：
41.2
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Onoe, Hiroaki;Okitsu, Teru;Takeuchi, Shoji
纤维状细胞构建体的人工重建可以极大地促进体外组织组装。在这里，我们表明，通过使用具有双同轴层流的微流体装置，可以制造封装ECM蛋白和分化细胞或体干细胞的米长的核-壳水凝胶微纤维，并且该微纤维重建活组织的内在形态和功能。我们还表明，这些功能性纤维可以组装，通过编织和缫丝，成宏观的细胞结构与各种空间模式。此外，包裹原代胰岛细胞并通过微导管移植到糖尿病小鼠肾包膜下空间的纤维使血糖浓度正常化约两周。这些微纤维可以用作重建纤维状功能组织的模板，这些功能组织模仿体内的肌肉纤维、血管或神经网络。
Artificial reconstruction of fibre-shaped cellular constructs could greatly contribute to tissue assembly in vitro. Here we show that, by using a microfluidic device with double-coaxial laminar flow, metre-long core-shell hydrogel microfibres encapsulating ECM proteins and differentiated cells or somatic stem cells can be fabricated, and that the microfibres reconstitute intrinsic morphologies and functions of living tissues. We also show that these functional fibres can be assembled, by weaving and reeling, into macroscopic cellular structures with various spatial patterns. Moreover, fibres encapsulating primary pancreatic islet cells and transplanted through a microcatheter into the subrenal capsular space of diabetic mice normalized blood glucose concentrations for about two weeks. These microfibres may find use as templates for the reconstruction of fibre-shaped functional tissues that mimic muscle fibres, blood vessels or nerve networks in vivo.