THE EFFECT OF DRUGS ON DIATOM VALVE MORPHOGENESIS
THE EFFECT OF DRUGS ON DIATOM VALVE MORPHOGENESIS
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DOI：
10.1007/bf01322985
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发表时间：
1989-01-01
期刊：
影响因子：
2.9
通讯作者：
PICKETTHEAPS, JD
中科院分区：
文献类型：
--
作者：
COHN, SA;NASH, J;PICKETTHEAPS, JD
用光学显微镜和扫描电子显微镜研究了不同药物对三种硅藻(Pinnularia spp.、Surirella robusta和Hantzschia inioxys)细胞壁(瓣膜)形态发生的影响。在瓣膜形成早期用微管(MT)干扰剂秋水仙碱治疗Surirella会导致瓣膜的典型畸形，由此正常周向的中缝通道的一部分在瓣膜表面形成异常凸出的嘴唇，最高可达.apprx。离阀门边缘20微米。这个畸形嘴唇的位置与加入秋水仙素时微管中心(MC)的位置一致，提示MC在瓣膜形成过程中可能在中缝管尖端的定位中发挥直接作用。在瓣膜形成的早期，这种MC向细胞顶端的迁移被代谢抑制剂2-4-二硝基苯酚(DNP)可逆地抑制。秋水仙碱对瓣膜形成的影响较轻，偶尔会引起中缝畸形，但侧向移位很小。在Hantzchia，秋水仙碱对中缝的位置没有影响，但长期暴露会导致中缝导管与瓣膜表面融合。Cochicine治疗还导致中缝中央交界处没有正常的曲率，以及该中央区域的异常孔洞形成。在Hantzschia早期瓣膜形成过程中加入细胞松弛素D会导致中缝管在瓣膜表面中心形成，这表明中缝管到瓣膜边缘的正常移位是肌动蛋白依赖的。对照和细胞松弛素处理的Hantzchia瓣膜的比较表明，瓣膜内的孔间距由相对于中缝的位置决定，而不取决于成熟瓣膜的侧面(外套层)或表面是否形成两个孔。
The effects of various drugs on cell wall (valve) morphogenesis was investigated in three species of diatoms (Pinnularia spp., Surirella robusta, and Hantzschia amphioxys) using light microscopy (LM) and scanning electron microscopy (SEM). Treatment of Surirella with the microtubule (MT) disrupting agent colchicine during early valve formation results in a characteristic malformation of the valve, whereby part of the normally circumferential raphe canal forms as an abnormal protruding lip on the valve surface, located up to .apprx. 20 .mu.m from the edge of the valve. The position of this malformed lip coincides with the location of a microtubule center (MC) at the time of colchicine addition, suggesting that the MC may play a direct role in positioning the tip of the raphe canal during valve formation. The migration of this MC to the tip of the cell during early valve morphogenesis is reversibly inhibited by the metabolic inhibitor 2-4-dinitrophenol(DNP). The effect of colchicine on Pinnularia valve formation is less severe, causing occasional malformation of the raphe, but little if any lateral displacement. In Hantzchia, colchicine has no effect on the positioning of the raphe, but prolonged exposure causes fusion of the raphe canal with the valve face. Cochicine treatment also results in the absence of the normal curvature at the central interruption in the raphe, as well as abnormal pore formation in this central area. Addition of cytochalasin D during early valve formation in Hantzschia causes the raphe canal to form in the center of the valve face, suggesting that the normal translocation of the raphe canal to the valve edge is actin-dependent. Comparison of valves from control and cytochalasin-treatment Hantzchia suggest that the pore spacing within the valve is determined by the position relative to the raphe, and does not depend on whether two pores form on the side (mantle) or the face of the mature valve.