Complement subcomponent C1q secreted by cultured human monocytes has subunit structure identical with that of serum C1q.
Complement subcomponent C1q secreted by cultured human monocytes has subunit structure identical with that of serum C1q.
复制标题
培养的人单核细胞分泌的补体亚成分C1q，其亚基结构与血清C1q相同。
DOI：
10.1042/bj2330451
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Volkin,DB
中科院分区：
文献类型：
--
作者：
Tenner,AJ;Volkin,DB
建立了酶联免疫吸附试验(e.l.i.s.a.)该方法能定量测定低至2 ng/ml的C1q浓度，并用灵敏的溶血试验研究了与人血清C1q发生交叉反应的物质的形态以及C1q在人单核细胞培养上清液中的溶血活性。这种物质在10-14天后在培养基中被检测到，并持续分泌到培养的第28天，在这一天培养终止。经琼脂糖抗C1q抗体特异性免疫吸附的细胞培养液经[~3H]Pro或[35S]蛋氨酸标记后，在SDS/PAGE上显示与血清C1q相同的多肽图谱。在非还原条件下，检测到两条蛋白质带，其Rf值与血清C1q、A-B和C-C二聚体的Rf值相同。还原时有三条明显的条带，与血清C1q的A、B、C链迁移一致。随着培养时间的延长，这些谱带中的放射性水平逐渐增加，这与E.I.S.A.一致。溶血C1q试验。将这些条带转移到硝酸纤维素膜上，可与单特异性抗C1q抗体反应。
An enzyme-linked immunosorbent assay (e.l.i.s.a.) that is capable of quantifying C1q concentrations as low as 2 ng/ml and a sensitive haemolytic assay were used to study the appearance of material that cross-reacts with human serum C1q as well as C1q haemolytic activity in human monocyte culture media. This material was detected in the medium after 10-14 days and continued to be secreted through to day 28 of culture, at which time the cultures were terminated. Material specifically immunoabsorbed with Sepharose-anti-C1q antibody from a culture medium of cells that was metabolically labelled with [3H] proline or [35S] methionine demonstrated a polypeptide pattern identical with that of serum C1q on SDS/polyacrylamide-gel electrophoresis. Under non-reducing conditions two protein bands were detected migrating with the same Rf values as the serum C1q A-B and C-C dimers. On reduction three bands were evident, which migrated identically with the A, B and C chains of serum C1q. The amount of radioactivity in these bands increased with time in culture, consistent with the e.l.i.s.a. and haemolytic C1q assays. These bands were reactive with monospecific anti-C1q antibody after transfer to nitrocellulose.