Candida Cell Heterogeneity Measured With a Microwave Flow Cytometer
Candida Cell Heterogeneity Measured With a Microwave Flow Cytometer
复制标题
DOI：
10.1109/jerm.2023.3239557
复制
发表时间：
2023-06
影响因子：
--
通讯作者：
Neelima Dahal;Carl Ehrett;Jeffrey A. Osterberg;R. Divan;Pingshan Wang
中科院分区：
文献类型：
--
作者：
Neelima Dahal;Carl Ehrett;Jeffrey A. Osterberg;R. Divan;Pingshan Wang
异质性是细胞群体的重要性质。它是生物进化背后的内在驱动力，也是有效疾病治疗的主要挑战，例如精确的固体癌症治疗。已经开发了各种“组学”方法、基于质谱的技术和流式细胞术用于细胞异质性表征。但是目前的方法要么是低通量的，要么是侵入性的。本工作中，我们研制了一种简单的微波流式细胞仪，用于单细胞C。和C.白色念珠菌是引起念珠菌血症疾病的两种最重要的念珠菌酵母。两个物种的细胞介电常数的散点图显示不同体积的细胞的共线性关系，这表明尽管细胞大小不均匀，但在很大程度上保守的细胞分子组成分布。并应用微波流式细胞仪检测抗真菌药物对念珠菌的作用。和C.白念珠菌细胞群，并观察药物诱导显著的异质性增加。结果表明，微波流式细胞术是一种高通量、无创的细胞异质性检测新方法。需要做更多的工作，包括确定所观察到的微波异质性的生物学意义。
Heterogeneity is an important property of a cell population. It is an intrinsic driving force behind biological evolution and a main challenge for effective disease treatment, such as precision solid cancer therapy. Various “omics” methods, mass spectroscopy-based techniques, and flow cytometry have been developed for cell heterogeneity characterization. But current methods are either low throughput or invasive. In this work, we develop a simple microwave flow cytometer to measure single C. tropicalis and C. albicans, which are two of the most important Candida yeasts that cause candidemia disease. The obtained scatter plots of cell permittivity for both the species show a colinear relationship for cells of different volumes, which indicates a largely conserved cell molecular composition profile despite heterogeneous cell sizes. We also apply microwave flow cytometer to measure antifungal drug effect on C. tropicalis and C. albicans cell population and observe that drugs induce significant heterogeneity increase. The results show that microwave flow cytometry is a promising new method for high throughput and noninvasive characterization of cell heterogeneity. More work is needed, including the determination of biological significance of the observed microwave heterogeneity.