High-throughput assay of (R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase
High-throughput assay of (R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase
复制标题
丙酮酸脱羧酶合成的 (R)-苯乙酰基甲醇的高通量测定
DOI：
10.1007/s00216-002-1579-1
复制
发表时间：
2002
影响因子：
4.3
通讯作者：
B. Lingen
中科院分区：
文献类型：
--
作者：
M. Breuer;M. Pohl;B. Hauer;B. Lingen
抽象的。建立了一种新的检测麻黄碱工业合成中手性中间体（R）-苯乙酰甲醇（R）-PAC的方法。它是丙酮酸脱羧酶催化苯甲醛生物转化的产物。该测定，使用2，3，5-氯化三苯基四唑，使高通量光度分析的酶的活性，从而避免了耗时的色谱程序。
Abstract. A novel assay has been developed for the detection of (R)-phenylacetylcarbinol, (R)-PAC, a chiral intermediate in the industrial synthesis of ephedrine. It is the product of a biotransformation of benzaldehyde catalysed by the enzyme pyruvate decarboxylase. The assay, using 2,3,5-triphenyltetrazolium chloride, enables high-throughput photometric analysis of the activity of the enzyme thus avoiding time-consuming chromatographic procedures.