Interaction between halothane and the nonadrenergic, noncholinergic inhibitory system in porcine trachealis muscle.
Interaction between halothane and the nonadrenergic, noncholinergic inhibitory system in porcine trachealis muscle.
复制标题
氟烷与猪气管肌中非肾上腺素能、非胆碱能抑制系统之间的相互作用。
DOI：
10.1097/00000542-199409000-00018
复制
发表时间：
1994
期刊：
影响因子：
8.8
通讯作者：
Hirshman,CA
中科院分区：
文献类型：
--
作者：
Lindeman,KS;Baker,SG;Hirshman,CA
BackgroundVolatile anesthetics significantly affect cholinergic neural transmission in the airways and relax airway smooth muscle. Activation of the nonadrenergic, noncholinergic inhibitory neural pathway, which is thought to be mediated by nitric oxide, relaxes human and procine airways. The purpose of the current study was to determine in the isolated porcine trachealis muscle whether relaxation of airway smooth muscle by halothane is mediated in part by activation of the nonadrenergic, noncholinergic inhibitory system.MethodsIsometric tension was measured in porcine trachealis muscle suspended in tissue baths in the presence of propranalol (10 (-6) M). After stimulation of postsynaptic nicotinic cholinergic receptors with 1, 1-dimethyl-4-phenyl-piper-azinium iodide (10 (-4) M) to prevent contractile responses to subsequent electrical field stimulation, carbachol (3 x 10 (-7) M) was added to increase tone. Nonadrenergic, noncholinergic relaxation responses to electrical field stimulation were then measured in the presence of inhibitors of nitric oxide synthase or L-arginine (the substrate for nitric oxide synthase), in the presence and absence halothane.ResultsElectrical field stimulation produced frequency-dependent relaxations that were attenuated by inhibitors of nitric oxide synthase (NG-nitro-L-arginine methyl ester [L-NAME] or NG-monomethyl-L-arginine, 10 (-4) M). Pretreatment with L-arginine (10 (-4) M) prevented the effect of L-NAME. Halothane (0.5% or 1.0%) neither enhanced nor attenuated nonadrenergic, noncholinergic relaxations in the presence of L-NAME, D-NAME, L-arginine, or D-arginine.ConclusionsHalothane, at concentrations< or= 1.0%, does not relax porcine airway smooth muscle in vitro by activating the nonadrenergic, noncholinergic inhibitory system.
