Foxi3 transcription factors and Notch signaling control the formation of skin ionocytes from epidermal precursors of the zebrafish embryo
Foxi3 transcription factors and Notch signaling control the formation of skin ionocytes from epidermal precursors of the zebrafish embryo
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DOI：
10.1016/j.ydbio.2007.04.044
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发表时间：
2007-07-15
影响因子：
2.7
通讯作者：
Hammerschmidt, Matthias
中科院分区：
文献类型：
--
作者：
Jaenicke, Martina;Carney, Thomas J.;Hammerschmidt, Matthias
离子细胞是参与维持渗透稳态的特化上皮细胞类型。在脊椎动物中，它们存在于肾脏系统中，而在低等脊椎动物的水生胚胎中，在皮肤中发现了额外的离子细胞。到目前为止，对离子细胞发育的机制知之甚少。在这里，我们证明，斑马鱼胚胎的皮肤离子细胞来自相同的前体细胞作为角质形成细胞。进行各种组合的增益和功能丧失的研究，我们表明，从表皮上皮细胞的分离是由Notch信号和两个叉头盒转录因子，Foxi3a和Foxi3b之间的相互作用。两个foxi3基因在离子细胞前体中表达，并且是Notch配体Jagged2a的离子细胞特异性表达和离子细胞分化所需的，其特征在于产生特定的ATP酶。离子细胞Notch配体反过来向邻近细胞发出信号，其中活化的Notch 1导致foxi3表达的抑制，使这些细胞成为角质形成细胞。将提出离子细胞与角质形成细胞发育的模型，假设迄今为止尚未确定的其他促离子细胞因子。(c)2007年爱思唯尔公司All rights reserved.
Ionocytes are specialized epithelial cell types involved in the maintenance of osmotic homeostasis. In amniotes, they are present in the renal system, while in water-living embryos of lower vertebrates additional ionocytes are found in the skin. Thus far, relatively little has been known about the mechanisms of ionocyte development. Here we demonstrate that skin ionocytes of zebrafish embryos derive from the same precursor cells as keratinocytes. Carrying out various combinations of gain- and loss-of-function studies, we show that the segregation of ionocytes from the epidermal epithelium is governed by an interplay between Notch signaling and two Forkhead-box transcription factors, Foxi3a and Foxi3b. The two foxi3 genes are expressed in ionocyte precursors and are required both for ionocyte-specific expression of the Notch ligand Jagged2a, and for ionocyte differentiation, characterized by the production of particular ATPases. Ionocytic Notch ligands, in turn, signal to neighboring cells, where activated Notch 1 leads to a repression of foxi3 expression, allowing those cells to become keratinocytes. A model for ionocyte versus keratinocyte development will be presented, postulating additional thus far unidentified pro-ionocyte factors. (c) 2007 Elsevier Inc. All rights reserved.