Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR
Detection and Quantification of BCR-ABL1 Fusion Transcripts by Droplet Digital PCR
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DOI：
10.1016/j.jmoldx.2013.10.007
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发表时间：
2014-03-01
影响因子：
4.1
通讯作者：
Joseph, Loren
中科院分区：
文献类型：
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作者：
Jennings, Lawrence J.;George, David;Joseph, Loren
实时定量RT-PCR监测BCR-ABL1融合转录物已成为慢性髓性白血病患者治疗的重要临床检测手段。然而，它在检测下限和定量上限方面存在一些固有的局限性。检测下限的提高可以帮助临床医生选择停止酪氨酸激酶抑制剂而不复发的候选人。定量限度的提高也可以避免不必要的检测或治疗方法的改变。在这里，我们展示了液滴数字RTPCR在简单，检测下限和定量限制方面的优势。我们期望液滴数字RT-PCR的优势将使其成为定量BCR-ABL1融合转录物的首选方法。
Monitoring BCR-ABL1 fusion transcripts by real-time quantitative RT-PCR has become an important clinical test for the management of patients with chronic myeloid leukemia. However, it has some inherent Limitations with regard to its lower limit of detection and limit of quantification. Improvement in the Lower limit of detection could aid clinicians in selecting candidates for discontinuation of tyrosine kinase inhibitors without relapse. Improvement in the limit of quantification may also avoid unnecessary testing or changes in therapy. Here, we demonstrate the advantages of droplet digital RTPCR with regard to simplicity, lower Limit of detection, and Limit of quantification. We expect the advantages of droplet digital RT-PCR wilt make it the preferred method for quantification of BCR-ABL1 fusion transcripts.