Surface IR immunosensors for label-free detection of benzo[a]pyrene.
Surface IR immunosensors for label-free detection of benzo[a]pyrene.
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DOI：
10.1016/j.bios.2010.08.031
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发表时间：
2010-12
影响因子：
12.6
通讯作者：
S. Boujday;S. Nasri;M. Salmain;C. Pradier
中科院分区：
文献类型：
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作者：
S. Boujday;S. Nasri;M. Salmain;C. Pradier
多环芳烃 (PAH) 是一类源自有机化合物不完全燃烧的污染物，存在于城市空气、水、土壤和食品中。由于其公认的致癌性，它们在城市空气中的浓度受到定期监测，政府部门也制定了关于其最大允许浓度的规则。苯并[a]芘（BaP）是PAHs之一，具有剧毒，其浓度与环境样品中PAHs总含量有很好的相关性；由于其分子量低且水溶性差，因此很少描述通过免疫传感器对其进行检测。在本文中，我们报告了使用偏振调制红外反射吸收光谱 (PM-IRRAS) 或带有耗散测量的石英晶体微天平 (QCM-D) 作为转导技术来测定 BaP 的光学和压电免疫传感器的设计和测试，并比较了它们的分析性能。我们发现与建立基于直接或间接竞争形式的免疫传感器相关。 PM-IRRAS 使用两种免疫传感器格式检测 BaP，灵敏度相似，约 100%。 5μM。所建立的间接竞争格式中的流动注射压电换能导致比 PM-IRRAS 所达到的灵敏度稍好。通过比较灵敏度，无法得出关于最有效设置的一般性结论，但报告的数据说明了 PM-IRRAS 以及流动注射 QCM-D 作为有效的无标记转导技术的潜力。
Polycyclic aromatic hydrocarbons (PAHs) are a family of contaminants originating from the incomplete combustion of organic compounds, present in the urban air, water, soil and also foodstuff. Due to their recognised carcinogenicity, their concentration in urban air is routinely monitored and governmental instances have set rules as regards their maximum allowable concentration. Benzo[a]pyrene (BaP) is one of the PAHs, highly toxic, whose concentration correlates well with the total PAHs contents in environmental samples; its detection by immunosensors has been rarely described because of its low molecular weight and poor solubility in water. In this paper, we report the design and testing of optical and piezoelectric immunosensors for the determination of BaP by using Polarisation-Modulation Infrared Reflection-Absorption Spectroscopy (PM-IRRAS) or Quartz Crystal Microbalance with dissipation measurements (QCM-D) as transduction techniques, and a comparison of their analytical performances. We found relevant to set up immunosensors based on a direct or indirect competitive format. BaP was detected by PM-IRRAS with both immunosensor formats with similar sensitivity, ca. 5μM. Flow-injection piezoelectric transduction in the indirect competitive format set up led to a slightly better sensitivity than that reached by PM-IRRAS. No general conclusion about the most efficient set up could be drawn from comparing the sensitivities, but the reported data illustrate the potential of PM-IRRAS as well as flow-injection QCM-D as efficient label-free transduction techniques.