Microarray analysis of cartilage: comparison between damaged and non-weight-bearing healthy cartilage
Microarray analysis of cartilage: comparison between damaged and non-weight-bearing healthy cartilage
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DOI：
10.1080/03008207.2019.1611797
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发表时间：
2019-05-31
影响因子：
2.9
通讯作者：
Bozkurt, Murat
中科院分区：
文献类型：
--
作者：
Asik, Mehmet Dogan;Gursoy, Safa;Bozkurt, Murat
目的：临床软骨病理中经常遇到局灶性软骨病变的有限愈合反应。本研究比较了同一例局灶性软骨病变患者软骨受损和未受损区域的基因表达模式。这项研究的目的是提供新的基因和蛋白质，这可能是未来潜在的研究目标。方法：在自体软骨细胞植入术（MACI）中，取10例膝关节局灶性软骨病变患者的软骨组织（健康非负重侧和损伤侧）。软骨退变状态根据ICRS标准进行表征。全基因组微阵列基因表达谱分析和一些差异调控基因用RT-PCR验证。结果：受损和未受损的非承重软骨表现出不同的基因表达谱。参与细胞信号传导、基质降解、缺氧和炎症反应的基因表现出显著的上调或下调。在局灶性病变中，HIF1 α、TIMP-2、EID1、EID2、NCOA3、NBR1、SP100和HSP90AA1等基因的表达与来自同一关节的健康非负重软骨相比显著升高，而TIMP-4的表达则较低。结论：本研究中检测的基因在局灶性软骨（ICRS 3-4）病变和同一关节的未损伤部位之间存在明显差异。我们相信，在这项研究中提出的数据可能用于临床目的，并在开发新的生物学治疗方法的指导。
Aim: A limited healing response to focal cartilage lesions is frequently encountered in the clinical cartilage pathology. This study compares the gene expression patterns of damaged and undamaged regions of cartilage obtained from the same patient with focal cartilage lesions. The aim of this study is to provide new genes and proteins, which may be a potential future target of research. Methods: During the autologous chondrocyte implantation (MACI) surgery, cartilage tissues (healthy non-weight bearing and Damaged-lesion side) were obtained from 10 patients with knee focal cartilage lesions. The degeneration status of the cartilage was characterized according to ICRS criteria. Whole genome microarray gene expression profiling was performed and some of the differentially regulated genes were validated with RT-PCR. Results: Damaged and undamaged non-weight bearing cartilage showed distinct gene expression profiles. Genes involved in cell signaling, matrix degradation, hypoxia, and the inflammatory response showed significant up- or down-regulation. In the focal lesions, expression of genes such as HIF1 alpha, TIMP-2, EID1, EID2, NCOA3, NBR1, SP100, and HSP90AA1 was significantly higher compared to healthy non-weight bearing cartilage from the same joint, whereas TIMP-4 was lower. Conclusion: The genes examined in this study differ distinctly between focal cartilage (ICRS 3-4) lesions and undamaged sites of the same joint. We believe that the data set forth in this study may be used for clinical purposes and be a guide in the development of new biological approaches for therapy.