Transplantation of testis germinal cells into mouse seminiferous tubules.
Transplantation of testis germinal cells into mouse seminiferous tubules.
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DOI：
10.1387/ijdb.9074943
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发表时间：
2003-02
期刊：
影响因子：
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通讯作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
中科院分区：
文献类型：
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作者：
T. Ogawa;J. Aréchaga;M. Avarbock;R. Brinster
在成年男性中，生殖细胞分化通过一个复杂的、高度组织化的和非常有效的过程在睾丸的曲细精管中发生。位于小管基底膜上的二倍体干细胞精原细胞群体持续进行自我更新并产生子代细胞，子代细胞启动细胞分化过程以产生成熟精子。每个睾丸都含有许多曲细精管，其两端连接到称为睾丸网的收集系统。成熟的精子从小管进入网，然后通过输出管进入附睾，在准备使卵子受精之前进行最后的成熟。在以前的研究中，我们已经证明，从可育小鼠收集的供体睾丸细胞移植到不育男性的曲细精管时能够产生精子发生。受体从供体来源的精原干细胞产生的精子能够使卵子受精并产生携带供体雄性单倍型的后代。此外，来自大鼠的供体睾丸干细胞在移植到小鼠睾丸后将产生正常的大鼠精子。精原细胞移植技术显然是有价值的，适用于许多物种，但它是困难的。因此，已经使用小鼠作为模型开发了几种将供体细胞引入受体的曲细精管的程序，并且在这里详细描述它们。结果表明，显微注射细胞悬浮液到曲细精管，输出管或睾丸网是同样有效的产生供体细胞来源的精子发生在受体。每种方法都可能对各种物种的不同实验目的有用。
In the adult male, germ cell differentiation takes place in the seminiferous tubules of the testis by a complex, highly organized and very efficient process. A population of diploid stem-cell spermatogonia that lie on the basement membrane of the tubule continuously undergoes self-renewal and produces progeny cells, which initiate the process of cellular differentiation to generate mature spermatozoa. Each testis contains many seminiferous tubules, which are connected at both ends to a collecting system called the rete testis. The mature spermatozoa pass from the tubules into the rete and are then carried through efferent ducts to the epididymis for final maturation before they are ready to fertilize an egg. In previous studies, we have demonstrated that donor testis cells collected from a fertile mouse are able to generate spermatogenesis when transplanted to the seminiferous tubules of an infertile male. The spermatozoa produced by the recipient from the donor-derived spermatogonial stem cells are able to fertilize eggs and produce progeny carrying the donor male haplotype. Furthermore, donor testis stem cells from a rat will generate normal rat spermatozoa following transplantation to a mouse testis. The spermatogonial transplantation technique is clearly valuable and applicable to many species, but it is difficult. Therefore, several procedures to introduce donor cells into the seminiferous tubules of a recipient have been developed using the mouse as a model, and they are described here in detail. The results indicate that microinjection of cell suspensions into the seminiferous tubules, efferent ducts or rete testis are equally effective in generating donor cell-derived spermatogenesis in recipients. Each approach is likely to be useful for different experimental purposes in a variety of species.