Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis
Circulating endothelial cells as a marker of ongoing vascular disease in systemic sclerosis
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DOI：
10.1002/art.20116
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发表时间：
2004-04-01
影响因子：
--
通讯作者：
Cortelezzi, A
中科院分区：
文献类型：
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作者：
Del Papa, N;Colombo, G;Cortelezzi, A
客观的。循环内皮细胞（CEC）在涉及血管损伤的不同条件下已有描述。血管异常在系统性硬化症（SSc）的发病机制中发挥着关键作用。本研究的目的是寻找 SSc 患者中是否存在 CEC，并评估其临床关联和可能的致病作用。方法。研究队列包括 46 名 SSc 患者和 40 名健康对照者。使用 FACScan 进行五参数、三色流式细胞术。 CEC被定义为CD45阴性、CD34阳性和P1H12阳性，激活的CEC被定义为CD45阴性和P1H12阳性、CD62阳性或CD106阳性。祖细胞被鉴定为CD34阳性和CD133阳性。结果。与健康对照相比，SSc 患者的总 CEC 计数和活化 CEC 计数显着较高，并且与疾病活动评分呈正相关。就内脏受累而言，CEC 数量与肺动脉高压严重程度之间存在显着相关性。在SSc患者中观察到高水平的内皮祖细胞，并且在疾病早期计数较高。结论。 SSc 患者中 CEC 的存在可能代表内皮疾病的直接证据，并且可能是活动性 SSc 的一个有前途的新临床标志物。值得注意的是，CEC 与肺动脉高压和一氧化碳弥散能力受损之间的关联仅在皮肤 SSc 有限的患者中很明显，这表明 CEC 在这种具有显着血管变化的疾病亚型中发挥着重要作用。循环内皮祖细胞的检测可能代表对早期 SSc 血管缺血的反应，作为血运重建的尝试。
Objective. Circulating endothelial cells (CECs) have been described in different conditions involving vascular injury. Vascular abnormalities play a key role in the pathogenesis of systemic sclerosis (SSc). The aim of this study was to search for the presence of CECs in patients with SSc and to evaluate their clinical associations and possible pathogenic role.Methods. The study cohort included 46 patients with SSc and 40 healthy controls. Five-parameter, 3-color flow cytometry was performed with a FACScan. CECs were defined as CD45 negative, CD34 positive, and P1H12 positive, and activated CECs were defined as CD45 negative and P1H12 positive, CD62 positive, or CD106 positive. Progenitors were identified as CD34 positive and CD133 positive.Results. Total and activated CEC counts were significantly higher in SSc patients compared with healthy controls and were positively correlated with the disease activity score. With respect to visceral involvement, significant correlation was observed between the CEC number and the severity of pulmonary hypertension. High levels of endothelial progenitors were observed in patients with SSc, and the counts were higher in the early stages of disease.Conclusion. The presence of CECs in patients with SSc may represent direct evidence of endothelial disease and may be a promising new clinical marker for active SSc. Notably, the association between CECs and pulmonary hypertension and impaired carbon monoxide diffusing capacity was evident in patients with limited cutaneous SSc only, suggesting an important role for CECs in this disease subset with prominent vascular changes. Detection of circulating endothelial progenitors may represent a response to vascular ischemia in early SSc, as an attempt at revascularization.