Adult cardiac progenitor cell aggregates exhibit survival benefit both in vitro and in vivo.
Adult cardiac progenitor cell aggregates exhibit survival benefit both in vitro and in vivo.
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DOI：
10.1371/journal.pone.0050491
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liao R
中科院分区：
文献类型：
--
作者：
Bauer M;Kang L;Qiu Y;Wu J;Peng M;Chen HH;Camci-Unal G;Bayomy AF;Sosnovik DE;Khademhosseini A;Liao R
使用外源性干细胞治疗性再生受损心肌的主要障碍仍然是植入细胞的存活率低。迄今为止，将干细胞递送到心肌中主要集中于细胞悬液的植入。我们假设，以聚集体形式递送祖细胞将有助于模拟具有适当细胞-细胞接触的内源性状态，并且可能有助于植入细胞的存活。微孔方法允许培养同质3D细胞聚集体，从而允许细胞-细胞接触。在这项研究中，我们发现在3D细胞聚集体中培养心脏祖细胞可以增加细胞存活，并保护细胞免受细胞毒素和应激源的影响，包括过氧化氢和缺氧/复氧诱导的细胞死亡。此外，使用心脏缺血再灌注损伤的小鼠模型，我们发现以3D聚集体形式递送心脏祖细胞改善了植入细胞的体内存活。总的来说，我们的数据支持这样的观点，即在3D细胞系统中的生长和细胞-细胞接触的维持改善了外源性细胞在递送到心肌中后的存活。这些方法可以作为一种策略，以改善心血管细胞为基础的治疗。
A major hurdle in the use of exogenous stems cells for therapeutic regeneration of injured myocardium remains the poor survival of implanted cells. To date, the delivery of stem cells into myocardium has largely focused on implantation of cell suspensions. We hypothesize that delivering progenitor cells in an aggregate form would serve to mimic the endogenous state with proper cell-cell contact, and may aid the survival of implanted cells. Microwell methodologies allow for the culture of homogenous 3D cell aggregates, thereby allowing cell-cell contact. In this study, we find that the culture of cardiac progenitor cells in a 3D cell aggregate augments cell survival and protects against cellular toxins and stressors, including hydrogen peroxide and anoxia/reoxygenation induced cell death. Moreover, using a murine model of cardiac ischemia-reperfusion injury, we find that delivery of cardiac progenitor cells in the form of 3D aggregates improved in vivo survival of implanted cells. Collectively, our data support the notion that growth in 3D cellular systems and maintenance of cell-cell contact improves exogenous cell survival following delivery into myocardium. These approaches may serve as a strategy to improve cardiovascular cell-based therapies.
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影响因子：
168.9
作者：
Makkar, Raj R.;Smith, Rachel R.;Cheng, Ke;Malliaras, Konstantinos;Thomson, Louise E. J.;Berman, Daniel;Czer, Lawrence S. C.;Marban, Linda;Mendizabal, Adam;Johnston, Peter V.;Russell, Stuart D.;Schuleri, Karl H.;Lardo, Albert C.;Gerstenblith, Gary;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
158.5
作者：
Birks, Emma J.;Tansley, Patrick D.;Yacoub, Magdi H.
通讯作者：
Yacoub, Magdi H.
影响因子：
4.9
作者：
Kang, Lifeng;Hancock, Matthew J.;Brigham, Mark D.;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
158.5
作者：
Narula, J;Haider, N;Khaw, BA
通讯作者：
Khaw, BA
DOI：
10.1073/pnas.0706760104
发表时间：
2007-08-28
影响因子：
11.1
作者：
Bearzi, Claudia;Rota, Marcello;Anversa, Piero
通讯作者：
Anversa, Piero