Neurons and glial cells differentially express P2Y receptor mRNAs in the rat dorsal root ganglion and spinal cord
Neurons and glial cells differentially express P2Y receptor mRNAs in the rat dorsal root ganglion and spinal cord
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DOI：
10.1002/cne.21066
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发表时间：
2006-10-01
影响因子：
2.5
通讯作者：
Noguchi, Koichi
中科院分区：
文献类型：
--
作者：
Kobayashi, Kimiko;Fukuoka, Tetsuo;Noguchi, Koichi
我们通过使用 31 个 S 标记的核糖核酸探针进行原位杂交组织化学 (ISHH)，检查了六种克隆大鼠 P2Y 受体亚型（P2Y1、P2Y2、P2Y4、P2Y6、P2Y12 和 P2Y14）的 mRNA 在背根神经节 (DRG) 和脊髓中的精确分布。在 DRG 中，所有神经元中 P2`Y1 和 P2Y2 mRNA 的表达率分别为 15% 和 24%。尽管每种受体均匀分布在神经丝阳性和神经丝阴性神经元之间，但 P2Y2 在 TrkA 阳性神经元中相当选择性地表达。雪旺细胞表达 P2Y2 mRNA，DRG 神经元周围的非神经元细胞（可能是卫星细胞）表达 P2Y12 和 P2Y14 mRNA。在 DRG 的任何细胞成分中均未发现 P2Y4 或 P2Y6 的 ISHII 信号。在脊髓中，一些背角神经元表达 P2Y1 和 P2Y4 mRNA，而腹角运动神经元则表达 P2Y4 和 P2Y6 mRNA。此外，灰质中的星形胶质细胞具有 P2Y1 mRNA，整个脊髓的小胶质细胞也表达 P2Y12 mRNA。推定的小胶质细胞 P2Y14 mRNA 表达较弱。鉴于缺乏针对每种 P2Y 受体亚型的高度选择性拮抗剂，这些发现应该为解释该领域的药理学和电生理学研究提供有用的信息。
We examined the precise distribution of mRNAs for six cloned rat P2Y receptor subtypes, P2Y1, P2Y2, P2Y4, P2Y6, P2Y12, and P2Y14, in the dorsal root ganglion (DRG) and spinal cord by in situ hybridization histochemistry (ISHH) with 31 S-labeled riboprobes. In the DRG, P2`Y1 and P2Y2 mRNAs were expressed by 15% and 24% of all neurons, respectively. Although each receptor was evenly distributed between neurofilament-positive and -negative neurons, P2Y2 was rather selectively expressed by TrkA-positive neurons. Schwann cells expressed P2Y2 mRNA, and the nonneuronal cells around the DRG neurons, perhaps the satellite cells, expressed P2Y12 and P2Y14 mRNAs. No ISHII signals for P2Y4 or P2Y6 were seen in any cellular components of the DRG. In the spinal cord, P2Y1 and P2Y4 mRNAs were expressed by some of the dorsal horn neurons, whereas the motor neurons in the ventral horn had P2Y4 and P2Y6 mRNAs. In addition, astrocytes in the gray matter had P2Y1 mRNA, and the microglia throughout the spinal cord expressed P2Y12 mRNA. P2Y14 mRNA was weakly expressed by putative microglia. These findings should provide useful information in interpreting pharmacological and electrophysiological studies in this field given the lack of highly selective antagonists for each P2Y receptor subtype.