Ringing the changes: emerging roles for DASH at the kinetochore-microtubule Interface
Ringing the changes: emerging roles for DASH at the kinetochore-microtubule Interface
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DOI：
10.1007/s10577-011-9185-8
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发表时间：
2011-04-01
影响因子：
2.6
通讯作者：
Millar, Jonathan B. A.
中科院分区：
文献类型：
--
作者：
Buttrick, Graham J.;Millar, Jonathan B. A.
着丝点和有丝分裂纺锤体之间的相互调节作用对于染色体分离的保真度至关重要。潜在有害的依恋在前中期和中期被纠正。当纺锤体产生的力量将染色体分离到相反的两极时，正确的附着必须在后期持续存在。在酵母中，异十聚体复合体在维持这一联系方面起着至关重要的作用。在体外，DASH可以形成低聚块和环，它们可以形成带有聚合和解聚微管尖端的承重附着物。在体内，DASH主要定位于着丝粒，在酿酒酵母和裂殖酵母中都具有维持纺锤体和染色体之间正确连接的作用。最近的工作已经开始描述DASH如何与外部动粒的其他组件一起作用，创建动态的、受调节的动粒-微管界面。在这里，我们回顾了一些关于DASH功能的关键实验，并讨论了它们对酵母和其他生物中动粒-微管连接的性质的影响。
Regulated interaction between kinetochores and the mitotic spindle is essential for the fidelity of chromosome segregation. Potentially deleterious attachments are corrected during prometaphase and metaphase. Correct attachments must persist during anaphase, when spindle-generated forces separate chromosomes to opposite poles. In yeast, the heterodecameric DASH complex plays a vital pole in maintaining this link. In vitro DASH forms both oligomeric patches and rings that can form load-bearing attachments with the tips of polymerising and depolymerising microtubules. In vivo, DASH localises primarily at the kinetochore, and has a role maintaining correct attachment between spindles and chromosomes in both Saccharomyces cerevisiae and Schizosaccharomyces pombe. Recent work has begun to describe how DASH acts alongside other components of the outer kinetochore to create a dynamic, regulated kinetochore-microtubule interface. Here, we review some of the key experiments into DASH function and discuss their implications for the nature of kinetochore-microtubule attachments in yeast and other organisms.