Naturally Occurring Fragments from Two Distinct Regions of the Prostatic Acid Phosphatase Form Amyloidogenic Enhancers of HIV Infection
Naturally Occurring Fragments from Two Distinct Regions of the Prostatic Acid Phosphatase Form Amyloidogenic Enhancers of HIV Infection
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DOI：
10.1128/jvi.06121-11
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发表时间：
2012-01-01
影响因子：
5.4
通讯作者：
Muench, Jan
中科院分区：
文献类型：
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作者：
Arnold, Franziska;Schnell, Jacqueline;Muench, Jan
精液是HIV-1传播的主要媒介。我们以前从精液中分离出前列腺酸性磷酸酶(PAP)的C-近端片段，它形成了淀粉样纤维，有效地增强了HIV感染。在这里，我们使用了相同的方法，并鉴定了另一种淀粉样多肽。令人惊讶的是，这种多肽来自PAP的N-近端片段(PAP85-120)，并形成与C-近端片段类似的正电纤维结构，增加病毒粒子与细胞的附着。我们的结果提供了由同一前体的不同区域的片段形成淀粉样蛋白的第一个例子，并进一步强调了淀粉样多肽在HIV传播中的可能重要性。
Semen is the major vector for HIV-1 transmission. We previously isolated C-proximal fragments of the prostatic acid phosphatase ( PAP) from semen which formed amyloid fibrils that potently enhanced HIV infection. Here, we used the same methodology and identified another amyloidogenic peptide. Surprisingly, this peptide is derived from an N-proximal fragment of PAP (PAP85-120) and forms, similar to the C-proximal fragments, positively charged fibrillar structures that increase virion attachment to cells. Our results provide a first example for amyloid formation by fragments of distinct regions of the same precursor and further emphasize the possible importance of amyloidogenic peptides in HIV transmission.