Cross-Reactivity of Palladium in a Murine Model of Metal-induced Allergic Contact Dermatitis
Cross-Reactivity of Palladium in a Murine Model of Metal-induced Allergic Contact Dermatitis
复制标题
DOI：
10.3390/ijms21114061
复制
发表时间：
2020-06-01
影响因子：
5.6
通讯作者：
Suzuki, Ryuji
中科院分区：
文献类型：
--
作者：
Shigematsu, Hiroaki;Kumagai, Kenichi;Suzuki, Ryuji
金属过敏通常通过斑贴试验诊断，但由于不同金属之间存在交叉反应，结果不一定反映临床症状。在这项研究中，我们建立了新的小鼠交叉反应性金属过敏模型，旨在从t细胞受体库的角度阐明免疫反应。将该模型分为两组：对镍敏化及钯基激发组和对铬敏化及钯基激发组。该模型出现海绵状水肿，炎症皮肤内和上皮周围浸润CD4(+) T细胞，类似于人类接触性皮炎。通过T细胞受体分析，我们在Ni和cr致敏pd小鼠中检测到高比例的T细胞携带Trav8d-1-Traj49和Trav5-1-Traj37。此外，粘膜相关的不变T细胞和不变自然杀伤T细胞也被检测到。我们的研究结果表明，携带Trav8d-1-Traj49和Trav5-1-Traj37的T细胞诱导了钯交叉反应性过敏的发生，并且粘膜相关的不变T细胞和不变自然杀伤T细胞也参与了不同金属之间的交叉反应。
Metal allergy is usually diagnosed by patch testing, however, the results do not necessarily reflect the clinical symptoms because of cross-reactivity between different metals. In this study, we established the novel mouse model of cross-reactive metal allergy, and aimed to elucidate the immune response in terms of T-cell receptor repertoire. This model was classified into two groups: the sensitization to nickel and challenge with palladium group, and the sensitization to chromium and challenge with palladium group. This model developed spongiotic edema with intra- and peri-epithelial infiltration of CD4(+) T cells in the inflamed skin that resembles human contact dermatitis. Using T cell receptor analysis, we detected a high proportion of T cells bearing Trav8d-1-Traj49 and Trav5-1-Traj37 in the Ni- and Cr-sensitized Pd-challenged mice. Furthermore, mucosal-associated invariant T cells and invariant natural killer T cells were also detected. Our results indicated that T cells bearing Trav8d-1-Traj49 and Trav5-1-Traj37 induced the development of palladium-cross reactive allergy, and that mucosal-associated invariant T and invariant natural killer T cells were also involved in the cross-reactivity between different metals.