Efficacy of chlorhexidine- and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro
Efficacy of chlorhexidine- and calcium hydroxide-containing medicaments against Enterococcus faecalis in vitro
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DOI：
10.1016/s1079-2104(03)00166-5
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发表时间：
2003-11-01
期刊：
影响因子：
--
通讯作者：
Friedman, S
中科院分区：
文献类型：
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作者：
Basrani, B;Tjäderhane, L;Friedman, S
Objective.我们试图评估氯己定（CHX）和氢氧化钙（Ca（OH）（2））在体外对粪肠球菌的疗效。通过琼脂扩散试验和体外人根接种法评价CHX（0.2%和2%凝胶或溶液）和Ca（OH）（单独或与0.2%CHX凝胶一起）的作用，以分别用光密度分析测量抑菌圈或细菌生长。用药7 d后收集感染根管标本，用脑心浸液培养24 h，进行光密度分析。在琼脂扩散试验中，CHX以浓度依赖性方式对粪肠球菌有效，但单独使用Ca（OH）（2）没有效果。在根管接种试验中，CHX对粪肠球菌的抑制作用显著高于Ca（OH）2（P
Objective. We sought to assess the efficacy of chlorhexidine (CHX) and calcium hydroxide, Ca(OH)(2), against Enterococcus faecalis in vitro.Study design. The effect of CHX (0.2% and 2% in gel or solution) and Ca(OH), (alone or with 0.2% CHX gel) was evaluated by using the agar diffusion test and an in vitro human root inoculation method, to measure zone of inhibition or bacterial growth with optical density analysis, respectively. For optical density analysis, samples from infected root canals were collected after 7 days of medication and were cultured for 24 hours in brain-heart infusion to detect viable bacteria.Results. In the agar diffusion test, CHX was effective against E faecalis in a concentration-dependent fashion, but Ca(OH)(2) alone had no effect. In the root canal inoculation test, CHX was significantly more effective against E faecalis than Ca(OH)(2) was (P