Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs
Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs
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DOI：
10.1016/j.vetmic.2014.09.002
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发表时间：
2014-11-07
影响因子：
3.3
通讯作者：
Yoon, K. J.
中科院分区：
文献类型：
--
作者：
Madson, D. M.;Magstadt, D. R.;Yoon, K. J.
猪流行性腹泻病毒（PEDV）与所有年龄的未感染猪的临床腹泻有关。本报告通过评估粪便病毒脱落、肠道病变的形态学分析和免疫组织化学染色的程度，描述了美国PEDV分离株感染后抗体产生和疾病进展的时间。将63头3周龄猪随机分为对照组（n = 27）和攻毒组（n = 36）。给发情的猪施用1 mL 1 x 10（3）PFU/mL US/爱荷华州/18984/2013 PEDV。通过口胃管饲法分离。在接种后第1、2、3、4、7天（dpi）对3头对照猪和4头攻毒猪进行尸检，此后每周进行一次尸检，直至dpi 35研究终止。在研究期间监测临床疾病、粪便脱落、体重和体温。在攻毒猪中观察到腹泻，从dpi 2开始，影响到dpi 6的大多数猪，到dpi 10消退。感染后1周，攻毒猪的平均日增重显著降低（P < 0.001）。在dpi 1时通过PCR在粪便中检测到PEDV，并且在猪的亚组中持续直到dpi 24。免疫组化（IHC）检测到PEDV特异性抗原的绒毛肠上皮细胞的攻击猪在dpi 1，2，3，4，7，和14。显微镜下病变包括重度弥漫性萎缩性肠炎，在dpi 3、4和7观察到绒毛长度显著减少（P < 0.001）。在本研究的条件下，PEDV的粪便脱落和IHC染色可以先于临床体征的观察并持续超过临床体征的观察，从而增加病毒传播的风险。(C)2014爱思唯尔有限公司版权所有。
Porcine epidemic diarrhea virus (PEDV) is associated with clinical diarrhea in naive swine of all ages. This report describes timing of antibody generation and disease progression following infection with a US PEDV isolate by assessing fecal viral shedding, morphometric analysis of intestinal lesions, and magnitude of immunohistochemical staining. Sixty-three, 3-week-old pigs were randomly allocated into control (n = 27) and challenged (n = 36) groups. Challenged pigs were administered 1 mL of 1 x 10(3) PFU/mL of US/Iowa/18984/2013 PEDV. isolate by oro-gastric gavage. Three control and four challenged pigs were necropsied on days post-inoculation (dpi) 1, 2, 3, 4, 7, and weekly thereafter, until study termination on dpi 35. Clinical disease, fecal shedding, body weight, and temperature were monitored during the study period. Diarrhea was observed in challenged pigs beginning for some on dpi 2, affecting a majority of pigs by dpi 6 and subsiding by dpi 10. Average daily gain was significantly lower (P < 0.001) for one week post-infection in challenged pigs. PEDV was detected in feces by PCR on dpi 1 and continued in a subset of pigs until dpi 24. PEDV-specific antigen was detected in villous enterocytes of challenged pigs by immunohistochemistry (IHC) on dpi 1, 2, 3, 4, 7, and 14. Microscopic lesions included severe diffuse atrophic enteritis with significantly reduced (P < 0.001) villous length observed on dpi 3, 4, and 7. Under the conditions of this study, fecal shedding of PEDV and IHC staining can precede and continue beyond the observation of clinical signs, thus increasing the risk of viral transmission. (C) 2014 Elsevier B.V. All rights reserved.