Oleic Acid Disorders Stratum Corneum Lipids in Langmuir Monolayers
Oleic Acid Disorders Stratum Corneum Lipids in Langmuir Monolayers
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DOI：
10.1021/la4002384
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发表时间：
2013-04-16
期刊：
影响因子：
3.9
通讯作者：
Walters, Russel M.
中科院分区：
文献类型：
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作者：
Mao, Guangru;VanWyck, Dina;Walters, Russel M.
众所周知，油酸(OA)会影响皮肤屏障的功能。本研究利用朗缪尔单分子膜及相关技术研究了模型角质层(SC)脂类与神经酰胺、胆固醇和棕榈酸(PA)之间的分子相互作用。将具有不同脂质组成的混合物分散在空气/水界面，并用表面压力-分子面积(pi-A)等温线跟踪其相行为。随着单分子膜中OA水平的增加，薄膜变得更具流动性和更好的可压缩性。由pi-A等温线得到的热力学参数表明，OA和SC之间存在优先相互作用，其混合物的成膜是热力学稳定的。分别用布鲁斯特角显微镜(BAM)和红外反射吸收光谱(IRRAS)研究了单层膜的结构和脂类构象。结果表明，较低浓度的OA优先与神经酰胺富集区混合并扰乱神经酰胺富集区，其次是较高浓度的OA干扰PA富集区和SC脂结构域分离。
Oleic acid (OA) is well-known to affect the function of the skin barrier. In this study, the molecular interactions between OA and model stratum corneum (SC) lipids consisting of ceramide, cholesterol, and palmitic acid (PA) were investigated with Langmuir monolayer and associated techniques. Mixtures with different OA/SC lipid compositions were spread at the air/water interface, and the phase behavior was tracked with surface pressure-molecular area (pi-A) isotherms. With increasing OA levels in the monolayer, the films became more fluid and more compressible. The thermodynamic parameters derived from pi-A isotherms indicated that there are preferential interactions between OA and SC lipids and that films of their mixtures were thermodynamically stable. The domain structure and lipid conformational order of the monolayers were studied through Brewster angle microscopy (BAM) and infrared reflection absorption spectroscopy (IRRAS), respectively. Results indicate that lower concentrations of OA preferentially mix with and disorder the ceramide-enriched domains, followed by perturbation of the PA-enriched domains and disruption of SC lipid domain separation at higher OA levels.