Distribution of cell layers in floral organs of chrysanthemum analyzed with periclinal chimeras carrying a transgene encoding fluorescent protein
Distribution of cell layers in floral organs of chrysanthemum analyzed with periclinal chimeras carrying a transgene encoding fluorescent protein
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用携带编码荧光蛋白的转基因的周周嵌合体分析菊花花器官中细胞层的分布
DOI：
10.1007/s00299-020-02518-y
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发表时间：
2020
影响因子：
6.2
通讯作者：
Naonobu Noda
中科院分区：
文献类型：
--
作者：
Ryutaro Aida;Katsutomo Sasaki;Satoshi Yoshioka;Naonobu Noda
关键信息使用携带编码荧光化合物的基因的转基因平周嵌合体，荧光蛋白可视化了菊花花器官细胞层的分布。摘要植物分生组织有三层细胞：最外层（L1）、第二层（L2）和内层（L3）。这些层在发育过程中得到维持，但对花器官内细胞层模式的细节了解有限。在这项研究中，我们可视化的细胞层的分布在菊花花器官的周缘嵌合体携带的基因编码的荧光化合物在L1或L2/L3层。花托、花瓣、花药、花丝、花柱、柱头、胚珠等器官的表皮细胞主要由L1层构成。雌蕊和胚珠内部的大部分传导组织也起源于L1。在杂交实验中，没有后代的L1嵌合体植物显示荧光，表明生殖细胞的菊花不是来自L1层。由于花青素色素只存在于花瓣的L1衍生的表皮细胞中，因此L1特异性基因整合可用于改变商业品种的花色，降低了转基因从转基因chromium流向野生亲缘植物的风险。
Key messageA fluorescent protein visualized distributions of cell layers in floral organs of chrysanthemum using transgenic periclinal chimeras carrying a gene encoding a fluorescent compound.AbstractPlant meristems have three cell layers: the outermost layer (L1), the second layer (L2), and the inner layer (L3). The layers are maintained during development but there is limited knowledge of the details of cell layer patterns within floral organs. In this study, we visualized the distributions of cell layers in floral organs of chrysanthemum using periclinal chimeras carrying a gene encoding a fluorescent compound in the L1 or the L2/L3 layers. The L1 layer contributed most of the epidermal cells of organs including the receptacle, petal, anther, filament, style, stigma, and ovule. The transmitting tissue in the pistil and most of the internal area of the ovule were also derived from the L1. In crossing experiments, no progeny of the L1-chimeric plants showed fluorescence, indicating that the germ cells of chrysanthemum are not derived from the L1 layer. Since anthocyanin pigment is present only in the L1-derived epidermal cells of petals, L1-specific gene integration could be used to alter flower color in commercial cultivars, with a reduced risk of transgene flow from the transgenic chrysanthemums to wild relatives.