Broadening cell selection criteria with micropallet arrays of adherent cells
Broadening cell selection criteria with micropallet arrays of adherent cells
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DOI：
10.1002/cyto.a.20424
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发表时间：
2007-10-01
期刊：
影响因子：
3.7
通讯作者：
Allbritton, Nancy L.
中科院分区：
文献类型：
--
作者：
Wang, Yuli;Young, Grace;Allbritton, Nancy L.
存在许多用于分离活的非粘附细胞的技术，其中分离决定通常基于细胞的荧光或免疫标记。分离贴壁细胞的方法以及扩大可能的分选标准的范围将具有很高的价值，并且是对现有策略的补充。在可释放托盘阵列上培养细胞。筛选阵列，并释放和收集单个细胞/托盘。传统的荧光和免疫标记的细胞是兼容的托盘阵列，是基于基因表达的分离。通过改变托盘的尺寸和在阵列上培养的细胞的数量，从异质群体中分离出单细胞或细胞克隆集落。由于细胞在整个分离过程中保持粘附，因此基于形态学特征（例如细胞形状）的分离是可行的。对阵列中的每个细胞进行重复测量，从而允许基于细胞的时间行为（例如生长速率）来选择细胞。托盘阵列系统提供了基于表型和时间标准以及替代方法无法获得的其他特征选择和收集贴壁细胞的灵活性。(C)2007年国际分析细胞学学会。
A host of technologies exists for the separation of living, nonadherent cells, with separation decisions typically based on fluorescence or immunolabeling of cells. Methods to separate adherent cells as well as to broaden the range of possible sorting criteria would be of high value and complementary to existing strategies. Cells were cultured on arrays of releasable pallets. The arrays were screened and individual cell(s)/pallets were released and collected. Conventional fluorescence and immunolabeling of cells were compatible with the pallet arrays, as were separations based on gene expression. By varying the size of the pallet and the number of cells cultured on the array, single cells or clonal colonies of cells were isolated from a heterogeneous population. Since cells remained adherent throughout the isolation process, separations based on morphologic characteristics, for example cell shape, were feasible. Repeated measurements of each cell in an array were performed permitting the selection of cells based on their temporal behavior, e.g. growth rate. The pallet array system provides the flexibility to select and collect adherent cells based on phenotypic and temporal criteria and other characteristics not accessible by alternative methods. (C) 2007 International Society for Analytical Cytology.