Spectral analysis methods for the robust measurement of the flexural rigidity of biopolymers.
Spectral analysis methods for the robust measurement of the flexural rigidity of biopolymers.
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用于稳健测量生物聚合物弯曲刚度的光谱分析方法。
DOI：
10.1016/j.bpj.2012.01.045
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发表时间：
2012
影响因子：
3.4
通讯作者：
M. Valentine
中科院分区：
文献类型：
--
作者：
David Valdman;P. Atzberger;Dezhi Yu;S. Kuei;M. Valentine
生物聚合物的机械性能可以通过统计分析它们在受到热力时所表现出的整体形状来确定。在实践中，由于低信噪比和其他伪影，从荧光显微镜图像中提取信息可能具有挑战性。为了解决这些问题，我们开发了一套用于图像处理和光谱数据分析的工具，该工具基于以正交多项式的光谱基础表示的生物聚合物轮廓表示。我们确定生物聚合物的形状和刚度使用全局拟合例程，优化效用函数测量的荧光强度重叠的量，这样的轮廓。这种方法允许过滤高频噪声和荧光中零星间隙的内插。我们使用基准测试来证明我们的方法的有效性，通过分析使用具有已知刚度的模拟生物聚合物生成的模拟图像的集合，并进行各种类型的图像噪声。然后，我们使用这些方法来确定紫杉醇稳定的微管的持久长度。我们发现，单个微管是很好地描述了蠕虫状链聚合物模型，和合奏的化学性质相同的微管显示显着的异质性弯曲刚度，这不能归因于采样或拟合误差。我们希望这些方法是有用的生物聚合物力学和相关的调节分子的影响的研究。
The mechanical properties of biopolymers can be determined from a statistical analysis of the ensemble of shapes they exhibit when subjected to thermal forces. In practice, extracting information from fluorescence microscopy images can be challenging due to low signal/noise ratios and other artifacts. To address these issues, we develop a suite of tools for image processing and spectral data analysis that is based on a biopolymer contour representation expressed in a spectral basis of orthogonal polynomials. We determine biopolymer shape and stiffness using global fitting routines that optimize a utility function measuring the amount of fluorescence intensity overlapped by such contours. This approach allows for filtering of high-frequency noise and interpolation over sporadic gaps in fluorescence. We use benchmarking to demonstrate the validity of our methods, by analyzing an ensemble of simulated images generated using a simulated biopolymer with known stiffness and subjected to various types of image noise. We then use these methods to determine the persistence lengths of taxol-stabilized microtubules. We find that single microtubules are well described by the wormlike chain polymer model, and that ensembles of chemically identical microtubules show significant heterogeneity in bending stiffness, which cannot be attributed to sampling or fitting errors. We expect these approaches to be useful in the study of biopolymer mechanics and the effects of associated regulatory molecules.
DOI：
10.1152/ajpheart.00132.2006
发表时间：
2006-09-01
影响因子：
4.8
作者：
Cooper, George
通讯作者：
Cooper, George