Dissection of placebo analgesia in mice: the conditions for activation of opioid and non-opioid systems
Dissection of placebo analgesia in mice: the conditions for activation of opioid and non-opioid systems
复制标题
小鼠安慰剂镇痛剖析：阿片类和非阿片类系统激活的条件
DOI：
10.1177/0269881109104848
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发表时间：
2010-10-01
影响因子：
4.1
通讯作者：
Luo, F.
中科院分区：
文献类型：
--
作者：
Guo, J-Y;Wang, J-Y;Luo, F.
Amanzio和Benedetti（J Neurosci 1999; 19：484-494）首先阐述了在人中激活阿片样物质和非阿片样物质安慰剂应答所必需的条件。本研究采用热板法研究了小鼠安慰剂镇痛作用是否分为阿片类和非阿片类成分。药物条件化通过条件线索刺激与非条件药物刺激的组合来进行，所述非条件药物刺激为阿片类激动剂盐酸吗啡或非阿片类阿司匹林。安慰剂镇痛反应是通过暴露于预先与药物条件配对的条件提示而诱发的。吗啡条件反射产生的安慰剂反应被纳洛酮完全拮抗。相比之下，阿司匹林调节后的条件提示引起了安慰剂效应，纳洛酮不能阻断。因此，我们首先在小鼠中诱发阿片类和非阿片类安慰剂反应，这些反应是纳洛酮可逆的或纳洛酮不敏感的，这取决于条件反射过程中使用的药物。这些发现支持安慰剂镇痛的机制可能取决于最初进行的药物调节。本小鼠程序可作为进一步了解安慰剂反应的阿片类和非阿片类机制的模型。
Amanzio and Benedetti (J Neurosci 1999; 19: 484-494) first addressed the conditions necessary for the activation of opioid and non-opioid placebo responses in human. Here, we investigated whether placebo analgesia is subdivided into opioid and non-opioid components in mice by using the model of hot-plate test. Drug conditioning was performed by the combination of the conditioned cue stimulus with the unconditioned drug stimulus, either opioid agonist morphine hydrochloride or non-opioid aspirin. Placebo analgesic responses were evoked by an exposure to a conditioned cue previously paired with drug conditioning. Morphine conditioning produced placebo responses that were completely antagonised by naloxone. By contrast, the conditioned cue after aspirin conditioning elicited a placebo effect that was not blocked by naloxone. Therefore, we first evoked opioid and non-opioid placebo responses in mice that were either naloxone-reversible or naloxone-insensitive, depending on the drug used in conditioning procedure. These findings support that the mechanisms underlying placebo analgesia may depend on the drug conditioning that was originally performed. The present procedure of mice may serve as a model for further understanding of the opioid and non-opioid mechanisms underlying placebo responses.