Biocompatible SU-8-Based Microprobes for Recording Neural Spike Signals From Regenerated Peripheral Nerve Fibers
Biocompatible SU-8-Based Microprobes for Recording Neural Spike Signals From Regenerated Peripheral Nerve Fibers
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DOI：
10.1109/jsen.2008.2006261
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发表时间：
2008-11-01
影响因子：
4.3
通讯作者：
Hughes, Gareth A.
中科院分区：
文献类型：
--
作者：
Cho, Sung-Hoon;Lu, Hong Meng;Hughes, Gareth A.
使用成熟的SU-8微加工技术构建的生物相容性神经微探针被设计用于记录周围神经再生轴突的纤维尖峰信号。这些微探针的特点是双极纵向金电极嵌入在“凹槽”的表面下，旨在引导沿凹槽长度再生轴突的生长，并限制与纵向电极接触的纤维数量。此外，使用人类皮肤成纤维细胞培养物筛选微探针的毒性，特别是这些SU-8微探针在神经界面应用中的生物相容性，特别使用周围神经中发现的两种敏感细胞类型的原代培养物进行了验证：纯化的雪旺细胞和外植的背根神经节（DRG）神经元及其纤维。SU-8微探针被手术植入一个独特的周围神经再生管内的大鼠坐骨神经。用这些SU-8微探针记录13只长期植入大鼠的长期纤维尖峰信号，时间为4 ~ 51周，没有任何组织损伤或炎症反应的迹象。
A biocompatible neural microprobe constructed using well-established SU-8 microfabrication techniques is described that was designed to record fiber spike signals from regenerated axons within peripheral nerves. These microprobes features bipolar longitudinal gold electrodes recessed below the surface within "grooves" designed to guide the growth of regenerating axons along the length of the grooves and limit the number of fibers that come in contact with the longitudinal electrodes. In addition, screening microprobe toxicity using cultures of human skin fibroblasts, the biocompatibility of these SU-8 microprobes for neural interface applications, in particular, was specifically verified using primary cultures of two sensitive cell types found in peripheral nerves: purified Schwann cells and explanted dorsal root ganglion (DRG) neurons and their fibers. The SU-8 microprobes were surgically implanted into transected rat Sciatic nerves within a unique peripheral nerve regeneration tube. Long-term fiber spike signals were recorded with these SU-8 microprobes in 13 chronically implanted rats for periods from 4 to 51 weeks without any signs of tissue damage or inflammatory reaction.