Analysis of Gut Microbiome Using Fecal Samples.
Analysis of Gut Microbiome Using Fecal Samples.
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使用粪便样本分析肠道微生物组。
DOI：
10.1007/978-1-0716-2549-1_21
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Ghannoum,Mahmoud
中科院分区：
文献类型：
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作者：
Gamal,Ahmed;Alabdely,Mayyadah;Steller,Kelly;Al-Shakhir,Hilmi;Elshaer,Mohammed;Ghannoum,Mahmoud
由于肠道微生物群与许多疾病有关，甚至为未来的治疗提供了可能性，多年来，它的研究一直受到人们的关注。然而，为了更深入地了解微生物组对我们健康的影响，拥有准确的微生物数据是至关重要的，这些数据在各个研究中都是可重复和可比较的。实验技术的差异，特别是DNA提取和测序方法的差异，可能会导致可变性和无法比较结果。在这一章中，我们详细描述了我们的微生物组分析方法，重点是粪便样本。
With the gut microbiome being implicated in many diseases and even offering the potential for future therapies, its study has been gaining interest over the years. However, to gain more in-depth insight and understanding of the impact of the microbiome on our health, it is critical to have accurate microbial data that is reproducible and comparable across studies. Variation in experimental techniques, particularly DNA extraction and sequencing methods, may result in variability and the inability to compare results. In this chapter, we describe in detail our microbiome analysis methodology focusing on stool samples.