Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in budded virus production and nucleocapsid assembly.
Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in budded virus production and nucleocapsid assembly.
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DOI：
10.1099/vir.0.050583-0
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发表时间：
2013-07
期刊：
影响因子：
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通讯作者：
Xingwei Xiang;Yunwang Shen;Rui Yang;Lin Chen;Xiaolong Hu;Xiaofeng Wu
中科院分区：
文献类型：
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作者：
Xingwei Xiang;Yunwang Shen;Rui Yang;Lin Chen;Xiaolong Hu;Xiaofeng Wu
家蚕核型多角体病毒（BmNPV）BmP 95是一个高度保守的基因，在迄今为止测序的所有杆状病毒基因组中发现，并且也在裸病毒中发现。为了研究BmP 95在病毒感染中的作用，在大肠杆菌中通过同源重组获得了一株BmP 95缺失病毒（vBmP 95-De）。荧光显微镜、光学显微镜和滴定分析表明，BmP 95缺失杆粒导致感染性出芽病毒（BV）的产生缺陷。然而，BmP 95的缺失并不影响病毒DNA的复制。电子显微镜显示，异常的管状结构的质量存在于转染BmP 95缺失杆粒的细胞中，表明BmP 95的缺失影响核衣壳的组装。这种缺陷可以通过将全长BmP 95插入BmP 95敲除杆粒的多角体蛋白基因座而不是BmP 95的N-末端结构域来挽救。总之，这些结果表明，全长BmP 95是必不可少的BV生产和所需的核衣壳组装。
Bombyx mori nucleopolyhedrovirus (BmNPV) BmP95 is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of BmP95 in virus infection in vitro, a BmP95 deletion virus (vBmP95-De) was generated by homologous recombination in Escherichia coli. Fluorescence and light microscopy and titration analysis indicated that the BmP95 deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of BmP95 did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the BmP95 deletion bacmid, indicating that deletion of BmP95 affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length BmP95 into the polyhedrin locus of the BmP95-knockout bacmid but not the N-terminal domain of BmP95. Together, these results showed that full-length BmP95 is essential for BV production and is required for nucleocapsid assembly.