Relationship between flow rate and NO production in postnatal mesenteric arteries.
Relationship between flow rate and NO production in postnatal mesenteric arteries.
复制标题
产后肠系膜动脉流速与 NO 产生的关系。
DOI：
10.1152/ajpgi.2001.280.1.g43
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Nowicki,PT
中科院分区：
文献类型：
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作者：
Reber,KM;Mager,GM;Miller,CE;Nowicki,PT
我们研究了3日龄和35日龄猪的肠系膜动脉弓，以确定灌流液流速和一氧化氮（NO）释放到肠系膜流出物之间的关系。肠系膜动脉弓在受控流条件下用蠕动泵使用温氧合Krebs缓冲液灌注。在体内流速（分别为9 ml/min和20 ml/min）灌注期间，3日龄和35日龄肠系膜中NO产生的基础速率分别为43.6 ± 4.2 nmol/min和12.1 ± 2.5 nmol/min。在3-但不是35天龄肠系膜中，NO产生率与宽范围流量（5-40 ml/min）的流量直接相关。两个年龄组都表现出了一个活跃的，虽然短暂的，响应于NO依赖性血管扩张剂P物质（10− 8 M/min）的输注NO产生的增加。酪氨酸激酶抑制剂除莠霉素A和L-精氨酸类似物-NMMA显著减弱了血流诱导的NO产生的增加，磷酸酶抑制剂氧化苯胂增加了3日龄血流诱导的NO产生的增加。细胞外Ca 2+的去除和细胞内Ca 2+商店的耗尽（无Ca 2 + Krebs与EGTA加毒胡萝卜素）在任何一组NO的产生没有影响。因此，基础率的NO生产是更大的肠系膜动脉弓从3日龄比从35日龄的猪，流量和NO生产率之间的直接关系是存在于肠系膜从3日龄，但不是35日龄，磷酸化事件是必要的，这种相互作用的发生。
We studied mesenteric arterial arcades from 3- and 35-day-old swine to determine the relationship between perfusate flow rate and release of nitric oxide (NO) into mesenteric effluent. Mesenteric arterial arcades were perfused under controlled-flow conditions with a peristaltic pump using warm oxygenated Krebs buffer. Basal rates of NO production were 43.6 ± 4.2 vs. 12.1 ± 2.5 nmol/min in 3- vs. 35-day-old mesentery during perfusion at in vivo flow rates (9 vs. 20 ml/min, respectively). Rate of NO production was directly related to flow rate over a wide range of flows (5–40 ml/min) in 3- but not 35-day-old mesentery. Both age groups demonstrated a brisk, albeit brief, increase in NO production in response to infusion of NO-dependent vasodilator substance P (10−8M/min). Tyrosine kinase inhibitor herbimycin A andl-arginine analogl-NMMA significantly attenuated flow-induced increase in NO production, and phosphatase inhibitor phenylarsine oxide increased magnitude of flow-induced increase in NO production in 3-day-olds. Removal of extracellular Ca2+and depletion of intracellular Ca2+stores (Ca2+-free Krebs with EGTA plus thapsigargin) had no effect on NO production in either group. Thus, basal rate of NO production is greater in mesenteric arterial arcades from 3- than from 35-day old swine, a direct relationship between flow rate and NO production rate is present in mesentery from 3- but not 35-day-olds, and phosphorylation events are necessary for this interaction to occur.