Inactivation of E. coli O157:H7 on Blueberries by Electrolyzed Water, Ultraviolet Light, and Ozone

Inactivation of E. coli O157:H7 on Blueberries by Electrolyzed Water, Ultraviolet Light, and Ozone
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DOI:
10.1111/j.1750-3841.2011.02595.x
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发表时间:
2012-04-01
影响因子:
3.9
通讯作者:
Hung, Yen-Con
Hung, Yen-Con
中科院分区:
农林科学3区
文献类型:
--
作者:
Kim, Chyer;Hung, Yen-Con

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由于蓝莓的营养和健康益处,人们对蓝莓的兴趣增加,导致消费量增加。然而,蓝莓大多是生食或最低限度加工的,像其他类型的新鲜农产品一样容易受到微生物污染。因此,本研究进行了评估的效果,静电喷雾的电解氧化(EO)水,紫外线,臭氧,臭氧和紫外线的组合在杀死大肠杆菌O 157:H7蓝莓。将E. coliO 157:H7接种于蓝莓花萼和果皮上,然后进行处理。静电EO水喷雾减少了大肠杆菌的初始种群。coliO 157:H7在蓝莓花萼和果皮上的接种量分别为0.13 ~ 0.24logCFU/g和0.88 ~ 1.10logCFU/g。4000 mg/L的臭氧处理降低了E. coliO 157:H7在蓝莓花萼和果皮上的生长量分别为0.66和0.72logCFU/g。20 mW/cm ~ 2的紫外光照射10 min是最有前途的单一技术,并实现了2.14和大于4.05的E. coli O 157:H7在蓝莓花萼和果皮上的表达。1分钟的臭氧和随后的2分钟的UV的组合处理实现了超过1和2个日志额外减少蓝莓花萼比单独的UV或臭氧,分别。
Increased interest in blueberries due to their nutritional and health benefits has led to an increase in consumption. However, blueberries are consumed mostly raw or minimally processed and are susceptible to microbial contamination like other type of fresh produce. This study was, therefore, undertaken to evaluate the efficacy of electrostatic spray of electrolyzed oxidizing (EO) water, UV light, ozone, and a combination of ozone and UV light in killing Escherichia coli O157:H7 on blueberries. A 5-strain mixture of E. coli O157:H7 were inoculated on the calyx and skin of blueberries and then subjected to the treatments. Electrostatic EO water spray reduced initial populations of E. coli O157:H7 by only 0.13 to 0.24 log CFU/g and 0.88 to 1.10 log CFU/g on calyx and skin of blueberries, respectively. Ozone treatment with 4000 mg/L reduced E. coli O157:H7 by only 0.66 and 0.72 log CFU/g on calyx and skin of blueberries, respectively. UV light at 20 mW/cm2 for 10 min was the most promising single technology and achieved 2.14 and greater than 4.05 log reductions of E. coli O157:H7 on the calyx and skin of blueberries, respectively. The combination treatment of 1 min ozone and followed by a 2 min UV achieved more than 1 and 2 log additional reductions on blueberry calyx than UV or ozone alone, respectively.