Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains
Intimin types determined by heteroduplex mobility assay of intimin gene (eae)-positive Escherichia coli strains
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DOI：
10.1128/jcm.01103-06
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发表时间：
2007-03-01
影响因子：
9.4
通讯作者：
Ratchtrachenchai, Orn-Anong
中科院分区：
文献类型：
--
作者：
Ito, Kenitiro;Iida, Mariko;Ratchtrachenchai, Orn-Anong
我们开发了一种快速遗传方法，通过针对内膜基因5‘保守区的异源双链迁移率分析(HNM)来筛选内膜基因的变体。EAE变异可分为4个主要HMA亚型和10个次要亚型。
We developed a quick genetic approach to screen variants of the intimin gene (eae) by using a heteroduplex mobility assay (HNM) that targets the 5' conserved region of eae. The eae variants were categorized into 4 major HMA types and 10 minor subtypes.