Behavioral analyses of mutants for two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila melanogaster
Behavioral analyses of mutants for two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila melanogaster
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DOI：
10.1266/ggs.83.257
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发表时间：
2008-06-01
影响因子：
1.1
通讯作者：
Matsuo, Takashi
中科院分区：
文献类型：
--
作者：
Harada, Eriko;Haba, Daisuke;Matsuo, Takashi
气味结合蛋白（OBP）在昆虫的化学感受中起作用。两个OBP基因Obp 57 d和Obp 57 e参与了果蝇独特的寄主植物偏好性的进化。对黑腹果蝇种组中Obp 57 dle基因组序列的比较分析表明，Obp 57 d和Obp 57 e的快速进化导致了这两个基因之间的功能分歧。这里，使用D.通过基因打靶产生的黑腹果蝇敲除菌株，我们研究了Obp 57 d和Obp 57 e在对一系列脂肪酸的行为反应中的作用。在基于味觉的产卵位点偏好性测定中，基因敲除果蝇比野生型果蝇表现出更强的对酸的偏好，表明Obp 57 d和Obp 57 e的正常功能与对C-6-C-9酸的正偏好性抑制有关。与野生型果蝇相比，每个敲除单倍型的杂合子也表现出显著不同的行为反应，表明Obp 57 d和Obp 57 e对行为具有基因剂量效应。相比之下，野生型和基因敲除果蝇在摄食试验和基于气味的自由行走试验中表现出相似的反应，表明两种OBP对摄食和嗅觉行为的贡献很小。结果表明，Obp 57 d和Obp 57 e在D.黑腹果蝇的OBP基因参与了果蝇生殖位点的决定，表明这两个OBP基因在果蝇的生态适应中起着重要作用。
The odorant-binding protein (OBP) functions in chemosensation in insects. Two OBP genes, Obp57d and Obp57e, are involved in the evolution of the unique hostplant preference in Drosophila sechellia. Comparative analysis of the Obp57dle genomic sequences in the Drosophila melanogaster species group has revealed that the rapid evolution of Obp57d and Obp57e has resulted in functional divergence between the two genes. Here, using D. melanogaster knockout strains generated by gene targeting, we examined the roles of Obp57d and Obp57e in behavioral response to a series of fatty acids. In the taste-based oviposition-site preference assay, the knockout flies showed stronger preference for acids than wild-type flies, indicating that the normal functions of Obp57d and Obp57e are associated with the suppression of positive preference for C-6-C-9 acids. Heterozygotes for each knockout haplotype also exhibited a significantly different behavioral response compared with wild-type flies, suggesting that Obp57d and Obp57e have a gene dosage effect on behavior. In contrast, the wild-type and knockout flies exhibited similar responses in the feeding assay and the odor-based free-walking assay, suggesting that the two OBPs' contribution to feeding and olfactory behaviors is small. Taken together, our results demonstrated that each of Obp57d and Obp57e in D. melanogaster contributes to the determination of reproductive sites, suggesting that the two OBP genes play an important role in ecological adaptation of Drosophila.