Interactions of soil metals with glomalin-related soil protein as soil pollution bioindicators in mangrove wetland ecosystems
Interactions of soil metals with glomalin-related soil protein as soil pollution bioindicators in mangrove wetland ecosystems
复制标题
土壤金属与球囊霉素相关土壤蛋白的相互作用作为红树林湿地生态系统土壤污染生物指示剂。
DOI：
10.1016/j.scitotenv.2019.136051
复制
发表时间：
2020-03-20
影响因子：
9.8
通讯作者：
Yan, Chongling
中科院分区：
文献类型：
--
作者：
Wang, Qiang;Lu, Haoliang;Yan, Chongling
通过矿物颗粒和元素的结合，球囊霉素相关的土壤蛋白（GRSP）在维持陆地土壤质量中起着关键作用，并有助于从陆地到水生生态系统的元素的命运。然而，关于GRSP在陆地和水生土壤中的金属螯合模式的知识很少，这限制了了解环境条件如何影响GRSP特征的进展。在这里，我们采用微观实验，以确定原始GRSP的分子组成来自三个丛枝菌根真菌，球囊霉intraradices，球囊霉versiforme和无梗囊霉laevis。为了深入了解环境GRSP的金属螯合模式，我们调查了中国南方主要的亚热带和热带红树林湿地。GRSP结合态重金属与重金属总量呈显著正相关，金属结合态对红树林土壤重金属的固定有贡献。傅里叶变换红外光谱结果表明，原始和环境GRSP馏分中含有羟基，羧基，酰胺和羰基官能团，这增强了金属结合。环境过程对GRSP的官能团类型没有影响，但显著改变了GRSP中官能团的相对含量。原始和环境GRSP的红外指纹分析表明，领域特异性，但没有分类特异性的GRSP的特征。对GRSP分子组成的生物统计分析进一步揭示了土壤污染源对与碳氢化合物、蛋白质、多糖和核酸相关的官能团含量比例的调节作用。利用GRSP红外指纹技术结合多元分析，建立了重金属污染源识别技术，为重金属污染源的识别提供了更可靠的依据。(C)2019 Elsevier B.V.版权所有。
Through binding of mineral particles and elements, glomalin-related soil protein (GRSP) plays a critical role in sustaining terrestrial soil quality and contributes to the fate of elements from terrestrial to aquatic ecosystems. There is little knowledge, however, of the metal sequestration patterns of GRSP in both terrestrial and aquatic soils, and this limits progress in understanding how environmental conditions influence GRSP characteristics. Here, we employed microcosm experiments to determine the molecular composition of original GRSP derived from three arbuscular mycorrhizal fungi, Glomus intraradices, Glomus versiforme and Acaulospora laevis. To gain insight into the metal sequestration patterns of environmental GRSP, we investigated major subtropical and tropical mangrove wetlands in southern China. GRSP-bound metals were significantly and positively correlated with total metals, and the metal binding contributed to the metal sequestration of mangrove soils. Fourier-transform infrared spectroscopy results showed that original- and environmental GRSP fractions contained hydroxyl, carboxyl, amide and carbonyl functional groups, which enhanced metal binding. Environmental process had no effect on the type of functional groups of the GRSP, while it significantly changed the relative content of the functional groups. The infrared fingerprint analyses of original- and environmental GRSP revealed field-specific, however, no taxon-specific characteristics of GRSP. Biostatistical analysis of the GRSP molecular composition further revealed that the soil pollution sources regulated the ratios of functional group contents associated with hydrocarbons, proteins, polysaccharides and nucleic acids. By GRSP infrared fingerprints coupled with multivariate analyses, we developed a technique for source identification of heavy metal pollution, giving more reliable evidence about contributing sources. (C) 2019 Elsevier B.V. All rights reserved.