Similarities among factors that render macrophages tumoricidal in lymphokine and interferon preparations.
Similarities among factors that render macrophages tumoricidal in lymphokine and interferon preparations.
复制标题
淋巴因子和干扰素制剂中使巨噬细胞具有杀肿瘤作用的因素之间的相似性。
作者：
R. Schultz;M. Chirigos
淋巴因子制剂，包括抗原刺激的卡介苗芽孢杆菌免疫脾细胞培养和不溶性豆豆蛋白A培养的正常脾细胞的上清液，与部分纯化的l细胞干扰素进行比较，以观察其在体外使静息巨噬细胞非特异性灭瘤的能力。抗原刺激淋巴因子和豆豆蛋白a诱导淋巴因子浓度分别低至0.5%和0.25%时，巨噬细胞被淋巴因子制剂显著激活。这些终点浓度均测定为每ml含有0.3单位的干扰素。未刺激的正常脾细胞、经豆豆蛋白a处理的nu/nu脾细胞或卡介苗芽孢杆菌免疫的脾细胞在缺乏致敏抗原的情况下获得的上清液不能增强巨噬细胞的杀瘤功能，而且缺乏干扰素。l细胞干扰素激活至少需要1单位/毫升。淋巴因子和干扰素制剂中含有的巨噬细胞活化因子在pH值2和56度时是稳定的，但在80度加热30分钟时被破坏，并被胰蛋白酶灭活。这些数据显示了这些不同的制剂诱导肿瘤杀伤巨噬细胞的共同特性。
Lymphokine preparations, including supernatants derived from antigen-stimulated Bacillus Calmette-Guérin-immune spleen cell cultures and normal spleen cells incubated with insoluble concanavalin A, were compared with partially purified L-cell interferon for the ability to render resting macrophages nonspecifically tumoricidal in vitro. Significant activation of macrophages by lymphokine preparations occurred at concentrations as low as 0.5 and 0.25% of the assay mixture for antigen-stimulated and concanavalin A-induced lymphokine, respectively. These end point concentrations were each determined to contain 0.3 unit of interferon per ml. Supernatants obtained from unstimulated normal spleen cells, concanavalin A-treated nu/nu spleen cells, or Bacillus Calmette-Guérin-immune spleen cells in the absence of sensitizing antigen did not enhance macrophage tumoricidal function and lacked interferon. Activation by L-cell interferon required at least 1 unit/ml. The macrophage-activating factors contained in lymphokine and interferon preparations were stable at pH 2 and at 56 degrees, but they were destroyed when heated at 80 degrees for 30 min, and were inactivated by trypsin. The data demonstrate common properties for the induction of tumoricidal macrophages by these divese preparations.