Using an indicator displacement assay to monitor glucose oxidase activity in blood serum.
Using an indicator displacement assay to monitor glucose oxidase activity in blood serum.
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DOI：
10.1021/ol070280o
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发表时间：
2007-03
期刊：
影响因子：
5.2
通讯作者：
Tianzhi Zhang;E. Anslyn
中科院分区：
文献类型：
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作者：
Tianzhi Zhang;E. Anslyn
使用先前发现的对葡萄糖酸具有高亲和力的硼酸受体，我们创建了一种比色指示剂置换试验（IDA），可以报告葡萄糖氧化酶（GOx）催化葡萄糖氧化产物的浓度。所得到的颜色变化直接反映了葡萄糖的浓度。然后，我们的传感系统成功地应用于测定人血清中的葡萄糖浓度，这提供了一种简便、比色、敏感和准确的葡萄糖测试。
[diagram: see text] Using a boronic acid receptor that was previously found to have high affinity for gluconic acid, we created a colorimetric indicator displacement assay (IDA) that can report the concentration of the product of glucose oxidase (GOx) catalyzed glucose oxidation. The color change obtained directly reflects the concentration of glucose. Our sensing ensemble was then successfully applied to determine the glucose concentration in human serum, which offers a facile, colorimetric, sensitive, and accurate glucose test.