Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine
Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine
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DOI：
10.1007/s11250-008-9228-0
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发表时间：
2009-04-01
影响因子：
1.7
通讯作者：
Bhattacharya, Mohan
中科院分区：
文献类型：
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作者：
Bandyopadhyay, Samiran;Sasmal, Debasis;Bhattacharya, Mohan
本研究旨在探讨印度阿鲁纳恰尔邦东北部丘陵牦牛区牦牛的布氏杆菌病血清阳性率。用抗生物素蛋白酶联免疫吸附试验（AB-ELISA）、孟加拉玫瑰红平板试验（RBPT）和标准试管凝集试验（STAT）检测374头动物，阳性率分别为23.79%、21.11%和18.98%。与AB-ELISA相比，STAT的相对敏感性和特异性分别为79.77%和100%，RBPT的相对敏感性和特异性分别为88.76%和100%。记录显示，感染率最高的是牦牛（31.42%），其次是小母牛（23.85%）和公牛（8.88%）。用标准剂量的牛种布氏杆菌19株疫苗对牦牛进行免疫后，免疫应答显示保护性抗体水平可持续210天。这是印度关于牦牛布鲁氏菌病流行和B 19株疫苗免疫的第一份报告。本研究为今后牦牛布鲁氏菌病的防治和根除提供了依据。
The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication programme of brucellosis in yaks.