Thrombin-Like and Factor X-Activator Components of Bothrops Snake Venoms
Thrombin-Like and Factor X-Activator Components of Bothrops Snake Venoms
复制标题
蛇毒中的类凝血酶和 X 因子激活剂成分
DOI：
10.1055/s-0038-1646781
复制
发表时间：
1979
影响因子：
6.7
通讯作者：
M. A. Barros
中科院分区：
文献类型：
--
作者：
L. Nahas;A. Kamiguti;M. A. Barros
在26种Bothrops物种中对蛇毒的凝血活性进行了比较研究，使用凝血酶样和因子X激活剂活性的特异性凝血系统。只有两个例外（B.黑色素瘤和B. Castelnaudi），所有毒液都显示出凝血酶样活性，因为它们能够直接凝结纤维蛋白原。B缺乏凝血酶样作用。黑刺红细胞毒液是由于纤维蛋白原溶解作用。五种毒液（B. atrox asper，B. bilineatus bilineatus、双线拟单胞菌B.科蒂亚拉湾，B. fonsecai和B. itapetiningae）在与血清、因子V和磷脂预孵育时不能产生凝血酶原激活剂。没有一种毒液似乎需要因子VII、VIII、IX、XII和XIII来完成其凝血作用。直接凝血酶原激活观察到在大多数的Bothrops毒液，单独或结合凝血酶样和因子X-激活剂的活动。B具有抗凝活性。卡斯特尔诺迪毒液，可能是由于一个。抗Xa作用。这项研究指出，蛇毒的凝血活性在同一属内不同，必须为每个物种的特点。因此，在Bothrops毒液中，凝血酶样和因子X激活剂成分并不总是相关的，凝血作用可能仅与其中一种成分有关。
Summary A comparative study on the coagulant activity of snake venoms was carried out in 26 Bothrops species, using specific clotting systems for the thrombin-like and the factor X- activator activities. With only two exceptions (B. erythromelas and B. castelnaudi) all venoms showed thrombin-like activity, since they were able to clot fibrinogen directly. The absence of thrombin-like action of B. erythromelas venom is due to a fibrinogenolytic effect. Five venoms (B. atrox asper, B. bilineatus bilineatus, B. cotiara, B. fonsecai and B. itapetiningae) were unable to produce a prothrombin activator when preincubated with serum, factor V, and phospholipid. None of the venoms seems to require factors VII, VIII, IX, XII and XIII for their complete coagulant action. Direct prothrombin activation was observed in most of the Bothrops venoms, alone or combined with thrombin-like and factor X-activator activities. An anticoagulant activity was exhibited by B. castelnaudi venom, probably due to an. anti- Xa action. This study points out that the coagulant activity of snake venoms varies within the same genus and must be characterized for each species. Thus, in Bothrops venoms the thrombin-like and factor X-activator components are not always associated, the coagulant effect may be related only to one of the components.