Inhibition of the adipose conversion of BALB/c 3T3 cells by 12-O-tetradecanoylphorbol-13-acetate: dependence on pH reduction via lactic acid production.
Inhibition of the adipose conversion of BALB/c 3T3 cells by 12-O-tetradecanoylphorbol-13-acetate: dependence on pH reduction via lactic acid production.
复制标题
12-O-十四烷酰佛波醇-13-乙酸酯抑制 BALB/c 3T3 细胞的脂肪转化：依赖于通过乳酸产生降低 pH 值。
DOI：
10.1002/jcp.1041040106
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发表时间：
1980
影响因子：
5.6
通讯作者：
Saladik,D
中科院分区：
文献类型：
--
作者：
O'Brien,TG;Saladik,D
研究了改变培养pH对BALB/c 3T3细胞脂肪转运的影响。我们先前已经证明，肿瘤促进剂12-0-十四酰佛波醇-13-乙酸酯(TPA)可以刺激细胞乳酸的产生，降低培养液的pH，并抑制该系统的分化。我们现在报道，在培养液中添加或有机酸，如D-或L-乳酸或醋酸，可通过降低pH，刺激细胞乳酸产生，抑制脂肪转化来模拟TPA的作用。通过改变NaHCO3浓度来降低pH值也抑制了分化。Dulbecco的改良最低基本培养基(DMEM)和最低基本培养基(MEM)的比较表明，前者的分化速度和程度都大于MEM，TPA既不抑制分化，也不刺激乳酸的产生。我们的标准MEM培养液可以使细胞对TPA的反应类似于DMEM，只需增加NaHCO3的浓度，从而使MEM的缓冲能力达到DMEM中的水平，这表明TPA只有在通过产生乳酸来降低培养pH值的情况下才能抑制该体系的分化。
The effect of modifying culture pH on the adipose convesion of BALB/c 3T3 cells was studied. We have previously shown that the tumor promoter 12‐0‐tetradecanoylphorbol‐13‐acetate (TPA) stimulates cellular lactic acid production, lowers the pH of the culture medium, and inhibits differentiation in this system. We now report that addition or organic acids such as D‐ or L‐lactic acid or acetic acid to the culture medium mimicked the effect of TPA by lowering pH, stimulating cellular lactic acid production, and inhibiting the adipose conversion. Lowering the pH by changing the NaHCO3concentration also inhibited differentiation.A comparison of Dulbecco's modified minimal essential medium (DMEM) vs minimal essential medium (MEM) indicated that in the former media, the rate and extent of differentiation was greater than in the MEM, and TPA neither inhibited differentiation nor stimulated lactate production. Our standard MEM medium could be made similar to DMEM with respect to the cells' response to TPA simply by raising the NaHCO3concentration, hence, the buffering capacity, of MEM to that present in DMEM, suggesting that TPA can only inhibit differentiation in this system if it can lower culture pH via lactate production.