Development and validation of a competitive immunoassay for urinary S-phenylmercapturic acid and its application in benzene biological monitoring
Development and validation of a competitive immunoassay for urinary S-phenylmercapturic acid and its application in benzene biological monitoring
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DOI：
10.1080/13547500110099663
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发表时间：
2002-03-01
期刊：
影响因子：
2.6
通讯作者：
Cocker, J
中科院分区：
文献类型：
--
作者：
Aston, JP;Ball, RL;Cocker, J
一种定量尿液s -苯基巯基酸（PMA）（一种苯特异性生物标志物）的免疫测定方法已被开发出来，其作为监测职业苯暴露的筛选工具的潜在用途已被证明。结果与气相色谱-质谱（GC/MS）数据的相关性（R = 0.92）证实了分析的可靠性。该试验已被配置为具有竞争力的酶联免疫吸附试验（ELISA），以促进样品的快速通量。ELISA的工作范围为40-1200 nmol l(1)尿液PMA，并且似乎不受结构相关尿液代谢物存在的影响。在非吸烟对照组中测量0-1.9 μ mol PMA/mol肌酐的背景水平（平均0.9 μ mol mol(1), n = 32）。通过在生物监测规划中应用该分析，确定了在不同工业过程中最近接触苯（8小时时间加权平均值- twa）在0-4.8 ppm范围内的情况。
An immunoassay that quantifies urinary S-phenylmercapturic acid (PMA), a benzene-specific biomarker, has been developed and its potential usefulness as a screening tool for monitoring occupational exposure to benzene has been demonstrated. Analytical reliability has been confirmed by correlation of results with gas chromatography-mass spectrometry (GC/MS) data (R = 0.92). The assay has been configured as a competitive enzyme-linked immunosorbent assay (ELISA) to facilitate rapid throughput of samples. The ELISA has a working range of 40-1200 nmol l(1) urinary PMA and appears to be unaffected by the presence of structurally related urinary metabolites. Background levels of 0-1.9 mumol PMA/mol creatinine (mean 0.9 mumol mol(1), n = 32) were measured in nonsmoking control subjects. Recent exposures to benzene (8 h time-weighted averages-TWA), during diverse industrial processes, over the range 0-4.8 ppm were identified by application of the assay in biological monitoring programmes.